Late infantile metachromatic
leukodystrophy: Clinical manifestations of five Taiwanese patients and Genetic features in Asia.
Looser chromatin has looser interactions with chromatin proteins, which can be easily displaced from the DNA in favor of metachromatic
binding of the dye to DNA phosphate groups (41).
This present study is aimed at comparing the commonly used metachromatic
stains viz., Wright-Giemsa, Toluidine blue, Methylene blue and Papanicolaou stain in the assessment of cell types of the oestrous cycle in rats.
Acetobacter indonesiensis bacteremia in child with metachromatic
leukodystrophy: an overview of current and prospective treatments," Bone Marrow Transplantation, vol.
Also, Wright-Giemsa staining showed that cells partially or completely lost the metachromatic
granules after activation (Figure 7).
At 2 years of age, comprehensive screening for metabolic disorders was completed including mannosidosis, fucosidosis, metachromatic
leukodystrophy, Sandhoff disease, lysosomal storage diseases, GM1 gangliosidosis, Krabbe disease, and mucopolysaccharidosis (types 13 and 6).
Toluidine Blue which has high affinity for the metachromatic
granules in the mast cells was chosen to specifically recognize these cells in the study samples.
The LSDs for which clinical trials are in various phases include Mucopolysaccharidosis Type-IIIA, Mucoploysaccharidosis Type-IVA, Mucoploysaccharidosis Type-VII, Metachromatic
Leukodystrophy, Wolman Disease, Alpha-mannosidosis and Niemann Pick C/D.
When she was only two, Abbie, the eldest of the three children, was diagnosed with metachromatic
leukodystrophy, a degenerative condition that reduces muscle tone.
Angela Moore Allan's daughter Laura has Metachromatic
, Leukodystrophy (MLD) which is a rare genetic disease of the brain.
Essentially, two grams of dehydrated algal tissue cut in small pieces were subjected to papain digestion (60[degrees]C, 24 hours) as previously described (Rodrigues et al., 2010), followed by fractionation of a sample of extract (20 mg) dissolved in 10 mL of 50 mM sodium acetate buffer (pH 5.0) by anion-exchange chromatography (DEAE-cellulose), where the column (1.2 X 12 cm)-bound SPs were eluted with NaCl (from 0 to 2 M, with 0.25 M of intervals) added to buffer of equilibrium and the collected fractions (2.5 mL) checked for SPs using metachromatic
assay containing dimethymethylene blue in an Amersham Bioscience Ultrospec 3100 spectrosphotometer at 525 nm (Farndale, Buttle, & Barrett, 1986).