It was observed that the presence of Mercaptoethanol
and SDS in the enzyme substrate system decreased pectinase activity significantly (Table 3).
In brief, the sputum samples were mixed with 2-3 volumes of the USP solution, which contained the following chemicals: 4-6 M guanidinum hydrochloride (a chaotropic agent which disrupts the hydrogen bonds), 50 mM Tris chloride (pH 7.5), 25 Mm Ethylene Diamine Tetra Acetic acid (EDTA), 0.5% sarkosyl, and 0.1-0.2 M Beta mercaptoethanol
These ingredients together brought about mucolysis and acted as detergents.
It has been reported that other antioxidant such as Beta- mercaptoethanol
, cysteine and cystine (Ali et al., 2003), and cysteamine (Gaspmini et al., 2000) added to IVM medium improve the rate of embryos development to the blastocyst stage.
Constant volume fractions (30 drops/fraction) were collected and counted on a Packard Auto-gamma counter to determine the elution position of radioactive MGP Controls were MGP and ovalbumin incubated with DSP, MGP alone, proteins incubated without DSP crosslinking, and DSP treated samples reduced with mercaptoethanol
to reduce and break crosslinks.
In this assay, superoxide anion is generated from molecular oxygen in the presence of EDTA, manganese (II) chloride, and mercaptoethanol
. Nicotinamide adenine dinucleotide phosphate oxidation is linked to the availability of superoxide anions in the medium.
Method 3 in combination with buffers 1 and 2, followed by LiCl and sodium acetate can remove the enlacing polysaccharides of nucleic acids for precipitation of RNA, while b- mercaptoethanol
helps to prevent oxidation reactions resulting in high integrity and amount of extracted RNA (Fig.
It was reconstituted, separately, in 1.5 mL of sample buffer (0.0625M Tris HCl pH 6.75, 2% SDS, 5% mercaptoethanol
, 10% glycerol and 0.001% bromophenol blue).
In the presence of denaturing agents such as SDS and mercaptoethanol
the separation of components depends on their molecular masses.
To eliminate protein contaminants, reagents such as proteinase K, ethylenediaminetetraacetic acid (EDTA), chloroform:isoamyl and mercaptoethanol
The use of high levels of PVP and P mercaptoethanol
in the clean up buffer had a considerable effect on neutralization of polyphenols and prevent oxidization of the secondary metabolites.
These were placed in 0.5ml of following medium: 10% (w/v) glycerol, 5% (v/v) 2 mercaptoethanol
and 2.3% (w/v) sodium dodecylsulfate (SDS), in 62.5 mM Tris/HCL buffer (pH 6,8).
There are many agents that could be used to reduce the [Cu.sup.II], including sodium sulfite, sodium hydrogen sulfite, inorganic salts comprising a metal ion, hydrazine hydrate, mercaptoethanol
, tetrahydrofuran, dihydroanthracene, 2,3-dimethylbutadiene, silane compounds, borane compounds, aldehydes, and derivatives of such compounds.