In the present study, the samples were not treated with mercaptoethanol
, which could have influenced dimerization and allowed the formation of oxidized polypeptides (homo dimers).
It has been reported that other antioxidant such as Beta- mercaptoethanol
, cysteine and cystine (Ali et al.
In contrast, the use of b- mercaptoethanol
helps to avoid oxidation reactions.
The use of high levels of PVP and P mercaptoethanol
in the clean up buffer had a considerable effect on neutralization of polyphenols and prevent oxidization of the secondary metabolites.
5ml of following medium: 10% (w/v) glycerol, 5% (v/v) 2 mercaptoethanol
, and 1% sodium sarkosyl) and incubated for 30 min at room temperature.
Mangani, The Complex of Bacillus Pasteurii urease with AY- mercaptoethanol
from X-ray data at 1.
Thus DTT or AY- mercaptoethanol
at low concentrations behaves as reducing agent.
Then the supernatant was removed and1 mL 70% ethanol and 5% mercaptoethanol
He used Mercaptoethanol
capping agent to prevent nanoparticles from being lumped through the process.
To eliminate protein contaminants, reagents such as proteinase K, ethylenediaminetetraacetic acid (EDTA), chloroform:isoamyl and mercaptoethanol
Preantral follicles were dissected out under stereo zoom microscope and washed thrice in preservation medium consisting of TCM-199 (90 %) supplemented with follicular fluid (10 %), Mercaptoethanol
(10 %) and gentamicin (50 [micro]g/ml oocytectomized follicles and were washed twice in culture medium before they were use).