denaturation temperature of DNA

(redirected from melting temperature of DNA)

de·na·tur·a·tion tem·per·a·ture of DNA

that temperature at which, under a given set of conditions, double-stranded DNA is changed (50%) to single-stranded DNA; under standard conditions, the base composition of the DNA can be estimated from the denaturation temperature, since the greater the denaturation temperature, the greater the guanine-plus-cytosine content (that is, GC content) of the DNA.
References in periodicals archive ?
The identified molecular properties of this compound that lead to its PCR-enhancing ability are based on (a) lowering the melting temperature of DNA and (b) thermostabilization of the Taq polymerase.
Dependence of the melting temperature of DNA on salt concentration.
Simplification of the empirical relationship between melting temperature of DNA, its GC content and concentration of sodium ions in solution.