Identification of Spata-19 new variant with exspression beyond meiotic phase
of mouse testis development.
The highest frequency (mean [+ or -] SD and percentages) was observed in stage III with 71 [+ or -] 5.7 (17.75%), followed by stage II with 60.5 [+ or -] 7.8 (15.12%), stage I with 59.5 [+ or -] 3.5 (14.87%), stage VII with 52 [+ or -] 2.8 (13%), stage VIII with 50.5 [+ or -] 3.5 (12.62%), stage VI with 49.5 [+ or -] 3.5 (12.37%), stage V with 29.5 [+ or -] 3.5 (7.37%), and stage IV (meiotic phase) with 27.5 [+ or -] 4.9 (12.37%).
Stages and phase of Mean [+ or -] SD Frequency seminiferous epithelium (%) Stage I 59.5 [+ or -] 3.5 14.87 Stage II 60.5 [+ or -] 7.8 15.12 Stage III 71.0 [+ or -] 5.7 17.75 Stage IV 27.5 [+ or -] 4.9 6.87 Stage V 29.5 [+ or -] 3.5 7.37 Stage VI 49.5 [+ or -] 3.5 12.37 Stage VII 52.0 [+ or -] 2.8 13.0 Stage VIII 50.5 [+ or -] 3.5 12.62 Premeiotic phase (stages I to III) -- 47.75 Meiotic phase (stage IV) -- 6.87 Postmeiotic phase (stages V to VIII) -- 45.37 TABLE 2: The diameter (mean [+ or -] SD) of germinal cell nuclei in eight stages (I-VIII) of seminiferous epithelium in the Javan muntjac during hard antler period.
At the age of 22-23 days, the most advanced germ cells are late spermatocytes (Clermont, 1972), allowing study of the whole meiotic phase
under our culture conditions (Staub et al., 2000).
It is not known how hormone-disrupting pollutants such as p,p'-DDE and PCBs interfere with the meiotic phase
, but infertile men often have an impaired chromosome synapsis and an increased frequency of chromosomes that are missing a recombination site (Martin 2006; Sun et al.
The results demonstrated that the expression of LDM during mouse oocyte meiotic maturation in vivo firmly rely on the meiotic phase
. As has been proposed, FF-MAS has the ability to trigger resumption of mouse oocytes meiosis, which can promote not only the nuclear but also plasma maturation (Marin Bivens et al., 2004b).
Knowledge of the meiotic phase
of the cell life cycle is important in understanding the radiosensitivity of the human oocyte.
The final extract was incubated for 45 min at 18 [degrees]C to initiate transition into the meiotic phase of the cell cycle.
Actin bundles detectable by AVEC-DIC microscopy assembled spontaneously in the meiotic phase extracts and formed interactive three-dimensional networks or pseudo-contractile rings by a mechanism of self-organization (6).
However, a genetic programme intrinsic to germ cells probably controls the progressive steps through mitotic, meiotic and post- meiotic phases
In some of these cells, the smallest bivalent showed a precocious separation, but exhibited a regular segregation in the subsequent meiotic phases