matrix-assisted laser desorption ionisation

matrix-assisted laser desorption ionisation

A process in mass spectrometry in which sample molecules are mixed with an excess of an energy-absorbing matrix and co-crystallised in a thin film on an inert metal support. Repeated irradiation of the film with a pulsed laser releases ions from the surface, which can be accelerated into a time-of-flight mass spectrometer. Because the matrix is usually a solid organic acid, the predominant mode of ionisation is protonation of the sample molecule M to form M H.
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Further analysis using matrix-assisted laser desorption ionisation time of flight mass spectrometry (MALDI-TOF MS) identified the causative organism as A.
Aggregatibacter aphrophilus misidentified as Brucella spp. in an immunocompetent patient with brain abscess
Matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) has recently been introduced into clinical microbiology laboratories in New Zealand and overseas.
A modified protocol for the direct identification of positive blood cultures by MALDI-TOF MS
Matrix-assisted laser desorption ionisation time-of-flight mass spectrometry for identification of clinically significant bacteria that are difficult to identify in clinical laboratories J.
MALDI-ToF: one lab's experience
The recommended tests include Dutch Qiagen's (ETR:QIA) Therascreen KRAS RGQ PCR Kit, German TIB MolBiol's KRAS LightMix Kit, as well as the methods pyrosequencing of codons 12, 13 and 61, and MALDI-TOF (matrix-assisted laser desorption ionisation time-of-flight) mass spectrometry of codons 12, 13 and 61.
NICE says yes to 4 tests for detection of KRAS mutation in colorectal cancer, no to 5 other similar products
The assays are utilised to determine hemoglobin variants and diabetes risk, using Bruker's Matrix-Assisted Laser Desorption Ionisation (MALDI) Biotyper platform for clinical mass spectrometry and based on intellectual property developed at the UVic GBC Proteomics Centre.
The new Matrix-Assisted Laser Desorption Ionisation time-of-flight mass spectrometry based test for hbA1c and genetic hemoglobin variants is intended for use on the MALDI Biotyper platform is likely to provide specificity, accuracy, speed of analysis and cost per analysis.
i"?Bruker collaborates with the University of Victoria-Genome British Columbia Proteomics Centre
It covers techniques such as electron and chemical jonisation and newer soft ion-isation techniques such as matrix-assisted laser desorption ionisation and electrospray.
MASS SPECTROMETRY OF NATURAL SUBSTANCES IN FOOD

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