We evaluated the expression of some key genes (nuclear factor kappa B (NF[kappa]B), matrix metalloproteinase 13
(MMP-13), a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4), collagen type I alpha 1 (cOl1A1), collagen type II alpha 1 (COL2A1), and cartilage oligomeric matrix protein (COMP)) implicated in joint homeostasis and pathology.
We also assessed RNA from IGF-1-transduced cells and control cells for mRNA transcript levels for the chondrogenic marker gene type II collagen alpha1 (Col2A1), as well as the hypertrophic marker type X collagen (Col10), matrix metalloproteinase 13
(MMP-13), and the osteogenic marker genes ALP and Osterix.
Partridge, "Sirtuin 1 is a negative regulator of parathyroid hormone stimulation of matrix metalloproteinase 13
expression in osteoblastic cells: role of sirtuin 1 in the action of PTH on osteoblasts," The Journal of Biological Chemistry, vol.
Loeser, "Stress-inducible nuclear protein 1 regulates matrix metalloproteinase 13
expression in human articular chondrocytes," Arthritis & Rheumatology, vol.
Bendele et al., "A new class of potent matrix metalloproteinase 13
inhibitors for potential treatment of osteoarthritis: evidence of histologic and clinical efficacy without musculoskeletal toxicity in rat models," Arthritis and Rheumatism, vol.
Interleukin-1 induction of col-lagenase 3 (matrix metalloproteinase 13
) gene expression in chondrocytes requires p38, c-Jun N-terminal kinase, and nuclear factor kappaB: differential regulation of collage-nase 1 and collagenase 3.