Singleplex qRT-PCR was performed for the selected marker genes
with certified QuantiTect primers from Qiagen (Venlo, The Netherlands) using 15 [micro]l final volume containing: 8.5 [micro]l SYBR green (BioRad 170-8880), 2.5 [micro]l of the QuantiTect forward/reverse primer mix, 2 [micro]l RNAse free water, and 2 [micro]l of 100x diluted cDNA.
acidocaldarius because marker genes
are lacking .
Perhaps the most ubiquitous application of high-throughput sequencing has been the amplification of conserved, phylogenedcally informative marker genes
from environmental DNA (typically genes encoding ribosomal RNA subunits or spacer regions, such as 16S for studies of Bacteria and Archaea, 18S for eukaryotic assemblages, and ITS for fungi).
The data presented here shows that the Cre/lox system is efficient for somatic and germinal excisions, and therefore extremely useful for marker gene
removal from transgenic rice.
Basically, if the antibiotic does not kill the gene-food, then the splicing has been successful because the inserted antibiotic-resistant marker gene
Blasted into the target cell along with the so-called desired gene is a "marker gene
," a gene conferring antibiotic resistance which helps determine if the desired gene was indeed inserted.
The marker gene
rol-6 and the green fluorescent protein (GFP) will be co-microinjected.
In order to facilitate the selective recovery of transformed cells, the mercury reductase genes were accompanied by flanking marker genes
the BAR (glufosinate resistance) gene and the GUSGFP visible marker gene
(which glows green under intense blue light).
The second will grant formal approval to Calgene's selectable marker gene
However, a petition to the FDA for an advisory opinion on a marker gene
used in the tomato is still pending.
The researchers analyzed the bacteria in fecal samples of 310 members of the Old Order Amish community in Lancaster County, Pa., using a process that enables them to identify a marker gene
that serves as a bar code for each type of bacteria.