Singleplex qRT-PCR was performed for the selected
marker genes with certified QuantiTect primers from Qiagen (Venlo, The Netherlands) using 15 [micro]l final volume containing: 8.5 [micro]l SYBR green (BioRad 170-8880), 2.5 [micro]l of the QuantiTect forward/reverse primer mix, 2 [micro]l RNAse free water, and 2 [micro]l of 100x diluted cDNA.
acidocaldarius because
marker genes are lacking [1].
Perhaps the most ubiquitous application of high-throughput sequencing has been the amplification of conserved, phylogenedcally informative
marker genes from environmental DNA (typically genes encoding ribosomal RNA subunits or spacer regions, such as 16S for studies of Bacteria and Archaea, 18S for eukaryotic assemblages, and ITS for fungi).
The data presented here shows that the Cre/lox system is efficient for somatic and germinal excisions, and therefore extremely useful for
marker gene removal from transgenic rice.
Basically, if the antibiotic does not kill the gene-food, then the splicing has been successful because the inserted antibiotic-resistant
marker gene provides protection.
Blasted into the target cell along with the so-called desired gene is a "
marker gene," a gene conferring antibiotic resistance which helps determine if the desired gene was indeed inserted.
The
marker gene rol-6 and the green fluorescent protein (GFP) will be co-microinjected.
In order to facilitate the selective recovery of transformed cells, the mercury reductase genes were accompanied by flanking
marker genes the BAR (glufosinate resistance) gene and the GUSGFP visible
marker gene (which glows green under intense blue light).
However, a petition to the FDA for an advisory opinion on a
marker gene used in the tomato is still pending.
The researchers analyzed the bacteria in fecal samples of 310 members of the Old Order Amish community in Lancaster County, Pa., using a process that enables them to identify a
marker gene that serves as a bar code for each type of bacteria.