marker exchange

marker exchange

The recombination of genes or marker cassettes between different chromosome locations, which can be done to probe the effect of spatial relationships between genes on their expression. Marker exchange occurs spontaneously in rec+ strains, and the plasmid can be readily eliminated or excluded. Marker exchange can be used to introduce chromosomal alleles into a plasmid.

Example
Bacterial genes that have been cloned on multicopy plasmids and subjected to mutagenisis may be returned to the chromosome by double homologous recombination events.
References in periodicals archive ?
fischeri vector pEVS179 to undergo transposon mutagenesis and ultimately antibiotic marker exchange, placing mutants back into the ES114 chromosome.