(2011) A complex of Pdi1p and the mannosidase
Htm1p initiates clearance of unfolded glycoproteins from the endoplasmic reticulum.
In humans, the N-linked glycans are processed by Golgi-resident mannosidase
IA, IB, and IC, which have different hydrolytic patterns but all generate [Man.sub.5]GlcN[Ac.sub.2].
Wang, "Production of lentiviral vectors with enhanced efficiency to target dendritic cells by attenuating mannosidase
activity of mammalian cells," Journal of Biological Engineering, vol.
Although in this case most of the mannosidase
recovered (88%) was readily soluble with little extra enzyme activity (12%) released on triton treatment.
Swainsonine is an inhibitor of lysosomal a-mannosidase, Golgi mannosidase
II and other calystegines that are potent glycosidase inhibitors, and it is suggested that these inhibitors disrupt intestinal glycosidases, lysosomal function, and glycoprotein processing, leading to enzymatic dysfunction and accumulation of complex oligosaccharides in the lysosomes of nervous and epithelial cells (COLEGATE et al., 1979; DORLING et al., 1980; STEGELMEIER et al., 2008).