The freely movable, light coloured and encapsulated mandibular salivary gland was located and differentiated form mandibular node
. The capsule of gland was incised and dorsomedially located auricular artery was located and ligated properly with 2/0 catgut.
After necropsy, small pieces ([less than or equal to] 0.5 cm) from brain, lungs, spleen, and mediastinal, tracheobronchial, and mandibular nodes from each pig were collected aseptically and maintained in RNA later solution (Ambion-Applied Biosystems, Life Technologies, USA) overnight at 4[degrees]C.
Significantly lower values (P < 0.05) were detected in TNF-[alpha] expression in blank group in mandibular nodes when compared with any of the vaccinated groups (Figure 1(f)), while in the remaining tissues these levels remained similar.