The freely movable, light coloured and encapsulated mandibular salivary gland was located and differentiated form
mandibular node. The capsule of gland was incised and dorsomedially located auricular artery was located and ligated properly with 2/0 catgut.
After necropsy, small pieces ([less than or equal to] 0.5 cm) from brain, lungs, spleen, and mediastinal, tracheobronchial, and mandibular nodes from each pig were collected aseptically and maintained in RNA later solution (Ambion-Applied Biosystems, Life Technologies, USA) overnight at 4[degrees]C.
Significantly lower values (P < 0.05) were detected in TNF-[alpha] expression in blank group in mandibular nodes when compared with any of the vaccinated groups (Figure 1(f)), while in the remaining tissues these levels remained similar.