IHC studies for WT1, Cytokeratin 7 (CK7) and ER showed positive reaction in atypical cells, while (Progesterone Receptor) PR, mammaglobin
and P53 showed negative reaction in tumor cells.
(21) CD138 is not helpful because it also stains a variety of carcinomas (22); therefore, MUM1 is a better plasma cell marker in this setting.23 For the distinction from breast cancer, estrogen receptor is almost always uniformly expressed in lobular breast cancer and can be a helpful diagnostic adjunct, particularly in combination with CK20 (positive in urothelial) and mammaglobin
(positive in breast).
An incisional biopsy was performed, and pathological examination revealed a poorly differentiated estrogen receptor-positive adenocarcinoma and a progesterone receptor-negative adenocarcinoma with focal cytoplasmic staining with mammaglobin
, supporting the possibility of parotid gland metastasis from breast carcinoma (Figure 1).
2 B and C: Lymph node: Tumor cells positive for Mammaglobin
and GCDFP-15 (100X)
Immunohistochemistry (IHC): EMA, CK7, ER, PR, MUC5AC, GCDFP15, Mammaglobin
, CK20--positive in tumor cells.
Both tumours are strongly positive for S100 protein, vimentin, mammaglobin
and cytokeratin markers (10).
Prognostic value of the molecular detection of circulating tumor cells using a multimarker reverse transcription-PCR assay for cytokeratin 19, mammaglobin
A, and HER2 in early breast cancer.
Raman candidates as diagnostic markers for breast cancer prognosis: mammaglobin
, palmitic acid and sphingomyelin," Analytica Chimica Acta, vol.
The immunohistochemical stains gave positive results for cytokeratin 7 (CK7), cytokeratin 8 (CK8), estrogen receptor (ER) (strong, 99%), progesterone receptor (PR) (strong, 99%) (Figure 5), Smad4 (DPC4), GAtA binding protein 3 (GATA-3), gross cystic disease fluid protein 15 (GCDFP-15), and mammaglobin
In addition, mammaglobin
, GATA3, and E-cadherin were found to be immunoreactive in the majority of tumor cells (Figure 5).
, a breast-specific gene, and its utility as a marker for breast cancer," Annals of the New York Academy of Sciences, vol.
Subsequent staining for breast carcinoma marker mammaglobin
was negative and evidence of ductal carcinoma in situ was absent.