The gels were stained for esterase (EST) as per the procedure of Glaszmann (1987), acid phosphatase (ACP) Vallejos (1983), alcohol dehydrogenase (ADH) and malate dehydrogenase
(MDH) Shaw and Prasad (1970).
(MDH) is used as a symbolic enzyme of the mitochondrial matrix .
was assayed by the method of Mehler et al.
, pyruvate kinase, and glucose-6-phosphate dehydrogenase activities were not affected significantly by dietary CBH levels.
MDH: Malate dehydrogenase
. LDH: Lactate dehydrogenase)
(MDH), a key enzyme in the tricarboxylic acid cycle (TCA), plays important metabolic roles in aerobic energy producing pathways and in malate shuttle (Figure 1) .
1.9), Glycerol-3-phosphate dehydrogenase (G3PDH, EC 22.214.171.124), Isocitrate dehydrogenase (IDH, EC 126.96.36.199), L-lactate dehydrogenase (LDH, EC 188.8.131.52), Malate dehydrogenase
(MDH, EC 1.1.
Of the enzyme systems initially assayed, alcohol dehydrogenase (ADH); lactate dehydrogenase (LDH); malate dehydrogenase
(MDH); phosphohexose isomerase (PHI/PGI); and 6-phosphogluconate dehydrogenase (6-PGD) resolved consistently.
The tested enzymes were aconitase (ACO), acid phosphatase (ACP), alcohol dehydrogenase (ADH), adenylate kinase (AK), arginine aminopeptidase (AMP), diaphorase (DIA), endopetidase (EP), esterase (EST), fructose-1,6-diphosphatase (FDP), glutamate dehydrogenase (GDH), glutamate oxaloacetate transaminase (GOT), hexokinase (HK), isocitrate dehydrogenase (ICD), leucine aminopeptidase (LAP), malate dehydrogenase
(MDH), malic enzyme (ME), peptidase with leucyl-alanine (PEP-LA), 6-phosphogluconate dehydrogenase (PGD), phosphoglucoisomerase (GPI), and shikimate dehydrogenase (SKDH) (Siciliano and Shaw, 1976; Vallejos, 1983; Van Geyt and Smed, 1984; Acquaah, 1992).
184.108.40.206), and sMDH-[A2.sup.*] (malate dehydrogenase
We scored nucleoside phosphorylase (Nsp), adenosine deaminase (Ada), and 6-phosphogluconate dehydrogenase (6-Pgd) on tris malate (pH 7.4); phosphoglucomutase (Pgm-1&2), tripeptide aminopeptidase (Pep-A&B), and malic enzyme (Mod) on tris citrate (pH 8.0); and lactate dehydrogenase (Ldh-1&2), glucose-6-phosphate isomerase (Gpi), and malate dehydrogenase
(Mdh-1&2) on amine citrate (pH 6.1).
Bands were revealed for the enzyme systems esterase (EST), according to Scandalios (1969), alcohol dehydrogenase (ADH), phosphoglucose isomerase (PGI), malate dehydrogenase
(MDH) and malic enzyme (ME), described by Alfenas (1991), and alpha amylase ([alpha] - AMY), according to Alfenas (1998).