3]@PMMA composite and the magnetic microspheres were dispersed in water by vigorous shaking, respectively; the Fe[(OH).
3]@PMMA composite microspheres and magnetic microspheres, respectively.
In conclusion, we have successfully prepared the magnetic microspheres with diameters in the range of 150-300nm.
Caption: Figure 1: Schematic illustration of the synthesis process of magnetic microspheres.
Goat anti-mouse IgG antibody-coated magnetic microspheres were sedimented by a magnet and washed twice in wash buffer (0.
6] Total TC is first sequestered from the serum sample or calibrator and isolated and concentrated by use of magnetic microspheres coated with anti-human TC mAb.
Two serum samples (400 [micro]L each) were mixed with 400 [micro]L of PBS and then treated with 40 [micro]L of a 10 g/L suspension of the mAb 3-9-coated magnetic microspheres or 40 [micro]L of a 10 g/L suspension of uncoated magnetic microspheres for 1 h at ambient temperature.
10] L/mol immobilized on magnetic microspheres assures that virtually all of the TC in serum or plasma will be sequestered without contamination by HC, which carries the major fraction of Cbl in the serum.