The [Fe.sub.3][O.sub.4] magnetic microspheres were synthesized following the procedure reported by Sun et al.
Buildup of [(Ti[O.sub.2]/POM).sub.n] Composite Films on Magnetic Microspheres. In order to prepare [(Ti[O.sub.2]/POM).sub.n] composite films, 0.2 g [Fe.sub.3][O.sub.4]@Si[O.sub.2] core-shell microspheres were first immersed in a poly(allylamine hydrochloride) (PAH) and poly(sodium 4-styrenesulfonate) (PSS) aqueous solution for 15 min to gain a surface with uniform charge.
Characterization of Magnetic Microspheres. The morphology of magnetic microspheres was monitored using a field emission scanning electron microscope (FESEM, model: JSM-6700F, JEOL, Japan) with an accelerating voltage of 10 kV.
The nitrogen adsorption-desorption isotherms of Fe[(OH).sub.3]@PMMA (a) and magnetic microspheres (b) were presented in Figure 5.
Figure 6 shows the TGA curves of Fe[(OH).sub.3]@PMMA composite microspheres and magnetic microspheres. After being calcined at 600[degrees]C, the Fe[(OH).sub.3]@PMMA composite nanosphere sample lost 80.5% of its weight in two steps (Figure 6(a)).
The Fe[(OH).sub.3]@PMMA composite and the magnetic microspheres were dispersed in water by vigorous shaking, respectively; the Fe[(OH).sub.3]@ PMMA composite results in a brown-colored suspension and it cannot be aggregated in the presence of an external magnetic field .
Lbbe and colleagues first applied magnetic polymer microsphere in clinical trials, they used magnetic microspheres (100 nm) containing epirubicin treating 14 patients with advanced stage hepatoma, and results showed that the method of cure reduces the side effect of drugs greatly and, at the same time, realized aggregation of microspheres in targeted area under the magnetic field.
Caption: FIGURE 5: Relationship between PLA/[Fe.sub.3][O.sub.4] magnetic microspheres concentration and hepatocyte HePG2 activity.
PLA coated [Fe.sub.3][O.sub.4] magnetic microsphere: the peak at 1180 [cm.sup.-1] represents the vibration of the C-O group, and another peak at 1759 [cm.sup.-1] corresponds to the C=O stretching vibration, maybe due to the LA molecule coating the surface of the [Fe.sub.3][O.sub.4] nanoparticles, so it further confirms the modification of the surface on magnetite nanoparticles by hydrophilic molecules, which facilitate the anisotropic crystal growth.
Encapsulated magnetic microspheres (EM1 100/40; mean diameter, 0.86 [micro]m) coated covalently with goat anti-mouse IgG (H+L) antibody were from Merck-Eurolab SAS.
IMMOBILIZATION OF THE mAb TO HUMAN TC ON MAGNETIC MICROSPHERES
Zhang, "Synthesis of [Fe.sub.3]04@Si[O.sub.2]@PMMA core-shell-shell magnetic microspheres
for highly efficient enrichment of peptides and proteins for MALDI-ToF MS analysis," Angewandte Chemie--International Edition, vol.