magnetic activated cell sorting

magnetic activated cell sorting

,

MACS

Technology used to separate unique populations of cells from an undifferentiated specimen of blood, bone marrow, or other tissue. It relies on the use of antibodies to specific cell types. A multicomponent specimen that contains some desirable cells is bathed in a solution of magnetically tagged antibodies. The cells of interest are attracted into a magnetic field and then collected for identification, experimentation, or therapeutic manipulation.
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Enrichment system with reporters, eGFP or H-2Kk as selection markers for fluorescence activated cell sorting (FACS) and magnetic activated cell sorting (MACS), respectively were constructed as previously described (Kim et al.
Monocytes were isolated from PBMC by magnetic activated cell sorting (MACS) beads according to the manufacturer's instructions (Miltenyi Biotec Inc.
A single, double, or triple density gradient is commonly used before separation strategies such as fluorescence-activated cell sorting (FACS) (9-12), magnetic activated cell sorting (13-15), immunomagnetic beads (16,17), antibody-conjugated columns (18), and charge flow separation (19,20).