Figure 2 shows the magic angle spinning
NMR spectra of peapods (I) and DWNTs (II).
Among the topics in this edition are using lipidic advances in imaging secondary ion mass spectrometry for biological samples, electron crystallography as a technique to study the structure of membrane proteins in a lipidic environment, insights from type II diabetes into the interplay of catalysis and toxicity by amyloid intermediates in lipid bilayers, biochemical and structural properties of the integrin-associated cytoskeleton protein talin, bioimage informatics for experimental biology, and determining the structure and dynamics of membrane proteins by magic angle spinning
solid-state nuclear magnetic radiation.
The Magic Angle Spinning
(MAS) of the samples leads to the complete averaging of the chemical shift anisotropy (CSA) and dipolar interactions: it follows that highly resolved spectra can be recorded even for powdered samples.
So even if a consensus could be reached on the ideal field strength, magic angle spinning
(MAS) rate, and pulse sequence, the present situation in which almost all SOM studies use a simple CP sequence and utilise whatever spectrometer is available to them regardless of field strength would likely prevail.
Advanced solid-state NMR probes such as MAS (magic angle spinning
) are a hybrid of probes used for liquid samples and those used for solid samples, and are useful for tissue analysis.
The 900 MHz instrument in Ottawa is the latest generation digital Avance II NMR spectrometer manufactured by Bruker BioSpin and is equipped with a number of solid-state NMR probes, for both magic angle spinning
(MAS) and static, capable of wide range of multinuclear NMR experiments.
The experimental conditions were as follows: 4 kHz magic angle spinning
, high power proton decoupling, 0.045 s acquisition time, 400 scans/spectrum, 75 [[micro]seconds] contact time, and 5 s recycle delay.
Although a large amount of material has been published on transcriptomics and proteomics, the other "omics" in the toxicologist's toolbox, these 12 discrete chapters constitute one of the most significant publications on metabonomics and includes such topics as biomarkers, NMR spectroscopy (including that of biofluids and "magic angle spinning
"), the application of metabonomics as an early in vivo toxicity screen, identification of endogenous and xenobiotic metabolites, data analysis, studying target organ toxicity, physiological variations in lab animals and humans, environmental applications of metabonomic profiling, and future developments.
In particular, they demonstrated that highly informative Si spectra of zeolites could be obtained by magic angle spinning
(MAS) experiments on a commercial high resolution NMR spectrometer.
Particular techniques driving solid state NMR analysis include high resolution magic angle spinning