in one experiment no organoids (1.) in one experiment growing crypt- like organoids (2.) Primary fetal Matrigel Monolayer and Mag/Ooc mix  growing organoids Primary fetal Uncoated Monolayered Sch II/Mz II mix (3) fibroblast, degrading cell conglomerates, no growing organoids [number of experiments]; (experiment number) Sch II: schizont 2nd generation, Mz II merozoite 2nd generation, Mag: macrogamont, Ooc: oocyst.
The success of development of macrogamonts and oocysts was directly connected with occurrence of these crypt-like structures.
If crypt-like organoids had developed up to four clusters of macrogamonts in 25-50% of the [micro]-slide, wells could be identified.
The mononuclear stages are probably young macrogamonts and the multinuclear stages fourth generation schizonts (Figure 3(h)).
This interpretation is supported by the occurrence of macrogamonts in only a part of the cell culture slide wells.
If merozoites IV are infecting new proper host cells, they would form even larger clusters of macrogamonts and this is shown in this study.
1, mature oocyst surrounded by outer layer (OL) and inner layer (IL) membrane and containing four sporocysts (SPC); 2, mature oocysts containing sporocysts (SPC) and free sporozoites (SP); 3, uninucleated (UN) and premature meront (PM); 4, infection of epithelial cell by two parasites, meront (M) and macrogamont (Ma), the host cell is displaced into the lumen but still attached to the underlying epithelium via a stalk- like structure.
Host cell infected with three parasites (arrowhead); 5, microgamont (MIC), macrogamont (Ma) and mature meronts (Me); 6, mature microgamonts (MM), attached to the underlying epithelium via a stalk-like structure (arrowhead), shedding microgametes, note disappearance of the parasite membrane and fragmentation of its surface; 7, macrogamontes (Ma) with wall-forming bodies (WF).
Electron micrographs of bile cells infected with different developmental stages of macrogamont. 12, the junction (arrowhead) between a displaced, infected and hypertrophied epithelial cell and the underling cell, host cell nucleus (HCN) and nucleolus (NU).
Macrogamonts were recognized by their nuclei each with a nucleolus and characterized by wall-forming bodies 1 and 2.
Mature macrogamonts with wall- forming bodies measured 13.8 - 14.2 x 10.6 - 11.2 (Fig.