Abbreviations AA: Ascorbic acid ACE: Angiotensin-converting enzyme AOE: Antioxidant enzymes CABG: Coronary artery bypass grafting CO: Carbon monoxide CYPs: Cytochrome P450 DGF: Delayed graft function DOX: Doxycycline HES: Hydroxyethyl starch HMP: Hypothermic machine perfusion
HO-1: Hemeoxygenase-1 HRUW: Hydrogen-rich UW solution HRW: Hydrogen-rich water [H.
tGST activity in kidney perfusate samples, collected hourly during machine perfusion
and stored at -20 [degree]C, was measured with an automated (Roche[R] Cobas Mira) spectrophotometric method, described by Habig and Jakoby (11), based on the conjugation of chlorodinitrobenzene at 25 [degree]C with glutathione to form glutathione Sdinitrobenzene, with the absorbance of the product measured at 340 nm.
Subnormothermic machine perfusion
for ex vivo preservation and recovery of the human liver for transplantation," American Journal of Transplantation, vol.
70 out of 336 kidney recipients in the machine perfusion
group developed delayed graft function (DGF) compared to 89 out of 336 in the cold storage group (adjusted odds ratio 0.
The clinical trial will compare outcomes in pairs of patients who receive contralateral pairs of donor kidneys, one preserved by machine perfusion
and the other by static storage, with left or right kidneys randomly assigned, the steering committee said in a press release.
The company says KPS-1 is an essential component of its kidney recovery system that uses proprietary machine perfusion
techniques for organ evaluation and therapy.