We have a demonstrated ability to use our immunoinformatics tools to screen and re-engineer protein therapeutics, in federally-funded, peer-reviewed programs (See FVIII, Botulinum toxin, Lysostaphin
programs for example) and commercial projects (See partnership with Biotest, AG).
In contrast, the absorbance of all cultures treated with lysostaphin
(a specific lytic enzyme for Staphylococci) dropped drastically early in the incubation period (within the first hour).
The answer was yes and the team quickly focused on lysostaphin
, an enzyme secreted by non-pathogenic Staph strains, harmless to humans and other organisms, capable of killing Staphylococcus aureus, including MRSA, and commercially available.
PEGylation also reduced the generation of antibodies against lysostaphin
protocols included lysozyme, lysostaphin
, and achromopeptidase from Sigma-Aldrich; proteinase K from Invitrogen; and a pyrimidine-specific RNase mixture, DNAse free (RNase, DNAse-free from Roche).
A University of St Andrews team, led by microbiologist Dr Peter Coote, found ranalexin had a "potent and significant" inhibitory effect on MRSA when combined with another antimicrobial compound, lysostaphin
Microbiologist Dr Peter Coote and his team combined it with an enzyme called lysostaphin
and discovered it had a deadly effect on the MRSA bug.
5% sodium lauryl sarcosine [Sarcosyl, Kodak International Biotechno-logies, New Haven, CT, USA], 1 mg/mL lysozyme [Life Technology, Sigma-Aldrich, Steinheim, Germany], and 7 [micro]g/mL lysostaphin
[Sigma]), then incubated overnight at 55[degrees]C in 4 mL Lysis 2 buffer (1% sodium lauryl sarcosine [Sarcosyl], 0.
After lysing the cells with lysostaphin
, and removing nucleic acids with DNAse and RNAse, the CP was separated from other cell components by DEAE Sephacel chromatography.
Researchers hope that Annie's mammary glands will secrete a protein called lysostaphin
that may help her resist attacks from S.
is the only anti-infective product that is able to both rapidly kill staphylococci (Staph) and eliminate staph biofilms.
Interestingly, Msa mutant gained resistant to lysostaphin
induced autolysis relative to wild type.