Isolated from such plants as Rivea corymbosa, Ipomoea violacea, and Ipomoea muricata, the bioactive alkaloid lysergol enhances the antimicrobial effects of different antibiotics, thus showing promise as an herbal bioenhancer (49).
Antibiotic pharmaceutical composition with lysergol as bio-enhancer and method of treatment.
Chemical constituents: Contains resin convolvulin, lysergol
, chanoclavine, penniclavine, elymoclavine (Khare, 2007).
Briefly, microtiter plate wells were precoated with human serum albumin to which lysergol
Coramine, 5-hydroxytryptamine, [alpha]-ergocryptine, ergonovine maleate, ergotamine tartarate, hordenine hemisulfate, L-tryptophan, lysergol, and N-demethyl-LSD (nor-LSD) were purchased from Sigma.
Compound % cross-reactivity 6-nor-LSD 52 Lysergic acid methyl-n-propyl amide (LAMPA) 34 2-oxo-3-hydroxy-LSD 3.4 iso-LSD 0.05 Ergonovine 0.016 Methylergonovine 0.008 D-Lysergic acid, lysergic acid amide Ergotamine, dihydroergotamine Methysergide, lysergol No cross-reactivity [alpha]-Ergocryptine, ergocristine Ergocornine, N,N-dimethyltryptamine (<0.008%) 5-Hydroxytryptamine, mescaline Psilocin, L-tryptophan Hordenine, coramine Gramine, L-amphetamine The degree of cross-reactivity was calculated from the concentration of substance that produced a decrease in signal that was equivalent to 0.5 [micro] g/L LSD.
A monoclonal antibody was previously generated to lysergol, the alcohol form of the lysergic moiety common to the ergot alkaloids.
Lysergol, lysergic acid, ergonovine, ergonovine maleate, ergovaline, ergotamine tartrate, ergocryptine, and 2-bromo-ergocryptine were tested for cross reaction in a competitive ELISA assay.
Cross reactivity of the anti-lysergol antibody from cell line 15F3 was similar to previous studies and had greater affinity to lysergol and lysergic acid than to the ergopeptine alkaloids (Hill et al., 1994) (Table 1).