We evaluated the effects of cAMP concentration on FRET levels in lysed
Cells were treated with TPA or EGF for 30 min, washed with ice-cold phosphate-buffered saline once, then lysed
with lysis buffer (2% SDS, 50 mM Tris-HCl pH 7.5).
Through the use of Boston Biomedica's patent pending, single-use PULSE Tubes, biological samples can be lysed
and the nucleic acids and proteins subsequently released.
Samples are lysed and then cooled at room temperature.
For example, 96 specimens and controls can be lysed at one time.
Antimicrobial susceptibility was tested by the microdilution method and haemophilus test medium with lysed
horse blood (7) with a commercially prepared dehydrated panel (Sensititre, Trek Diagnostic System, East Grinstead, UK) as previously described (8).
The results were expressed as the percentage of ATP released relative to the total ATP present in cells lysed by means of digitonin (50 [micro]M) (16).
In the presence of fibrinogen (1 mg/mL), Ni[Cl.sub.2] induced platelet aggregation (1 mM: 46.7 [+ or -] 9.6%; 5 mM: 78 [+ or -] 8.7%) (Figure 1) and released ATP from the internal stores (25.4 [+ or -] 4.5% of the total ATP present in lysed cells vs.
Chloramphenicol and penicillin MICs were determined for all isolates, according to the recommendations of the National Committee for Clinical Laboratory Standards, by the broth microdilution method using Mueller-Hinton broth with 5% lysed
horse blood incubated in 5% [CO.sub.2] (5).