Specializing in aseptic manufacturing and filling of parenterals, Recipharm in Masate, located close to Milan, offers lyophilisation
capabilities in both vials and ampoules.
The adaptations of lyophilisation
and sonication made to the gravity and then vacuum assisted MSPD extraction method improved minimum recovery by approximately 18%.
The new extended 4-day training courses have been designed to enable people involved with lyophilisation
, at all levels of experience, to gain considerable benefit and lo reinforce their classroom training with extensive laboratory work.
Darrington, "Developments in Laboratory Scale Lyophilisation
for Purification Laboratories," Genevac (2006).
The centralized laboratory system is access controlled and composed of dedicated areas for biochemical and haematological analysis, bacteriology, serology, tissue culture, molecular biology, HIV, lyophilisation
and strain maintenance, etc.
In some cases, filtrates were concentrated to 50X by lyophilisation
followed by reconstitution and dialysis before testing.
MannKind worked with Cryogenic Equipment Services to modify the cryopelletizer to create uniform pellets from the slurry so that the water could be removed quickly and consistently during the bulk lyophilisation
The CMO is able to fill bulk volumes under 100 litres, including products that require aseptic liquid filling and lyophilisation
Rizvanov et al., "Recent advances and future direction in lyophilisation
and desiccation of mesenchymal stem cells," Stem Cells International, vol.
Fresh and dry weights were recorded before and after lyophilisation
and were subsequently ground into fine powder.