Test was performed in white half area 96 well plates [Costar, NY, USA], which was incubated at 37AoC for 15 min in the thermostat chamber of luminometer
[Labsystems, Helsinki, Finland].
Test was performed in half zone (white) 96-well plates (Costar, NY, USA), which was incubated for 15 min at 37 AdegC, in the indoor regulator chamber of luminometer
(Labsystems, Helsinki, Finland).
In the presence of the substrate furimazine, this results in a bioluminescent signal that can be readout with a standard luminometer
. We initially applied our bioassays on urine samples because of (a) the anticipated higher concentrations in urine, (b) the fact that many phase I SC metabolites retain activity at CB receptors, and (c) the combined presence of distinct active metabolites is likely to be beneficial for the assays' sensitivity.
Plates were incubated at 30[degrees]C with continuous stirring at 300 rpm, and bioluminescent activity was measured in relative light units (RLU) using a luminometer
(FLUOstar Omega Microplate Reader, BMG Labtech, Offenburg, Germany) at 15min intervals for at least 5 hours.
Each culture grown overnight in pH 5.5 and pH 7 LB was incubated with plain Sepharose beads or mannose-coated Sepharose beads with or without 2% mannose (wt/vol) with rocking and then tested for bioluminescence using a FB 12 bioluminescence single tube luminometer
GloSensor luciferase activities were measured on a microplate luminometer
(Berthold Centro XS3 LB960).
Serum cell free DNA was analyzed by luminometer
after the nucleic acid staining protocol of SYBR Gold Nucleic Acid Gel Stain and serum HMGB-1 concentration was measured using HMGB1-ELISA kit.
The emitted light intensity is linearly related to the ATP concentration and it is measured using a luminometer
. To perform cytotoxicity tests, cells were recovered, counted and adjusted to the concentration of 2 x [10.sup.5]/mL, seeded in a flat bottom 96-well culture plate, and incubated until monolayer formation.
This economical and versatile microplate luminometer
system is compatible with ALPCO's line of STELLUX[R] Chemiluminescent assays as well as with any assay that can be run on a standard glow plate reader.
Luminescence counts were obtained by using a microplate luminometer
(Hidex Microplate Luminometer
Penshaped test devices contain a modified firefly enzyme that reacts with organic material to produce light, which is then measured in a handheld meter (called a luminometer
) about the size of a TV remote control.
is used to test for ATP and will show how well an area was cleaned and disinfected.