Tissues were placed into a 2 mL phosphate bufer solutions-4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (PBS-Hepes), and luminol
(0.2 mM) and lucigenin (0.2 mM) were added to the two tubes (4 mL for each tube), which included the samples of the same tissue.
0.5 mL sample with different concentrations was added to 4 mL luminol
solution, and the mixture was put into detection pool.
We thus assessed the in vivo ROS level in a comprehensive way with luminol
Del Castillo, "Luminol
luminescence induced by 2,2'-azo-bis(2-amidinopropane) thermolysis," Free Radical Research, vol.
The in vitro antioxidant activity (AA%) of Citrus-nAg was assayed through chemiluminescence method on a Chemi-luminometer Turner Design TD 20/20 (USA), by using the system containing: luminol
(1 mM), Tris-HCl buffer solution (pH 8.6), and [H.sub.2][O.sub.2] (10 [micro]M), as free radical generator.
(a) Mixtures were prepared using 1ml of Hank's solution, containing luminol
0.8 x [10.sup.-4] M, 4U HRP, and lactobacillus bacteria at different MOI (1: 1 and 1: 500; bacteria : macrophages).
Cell suspension (3.0 x [10.sup.5]) was incubated with 50 [micro]l of the samples with tested compounds in the proper concentration and luminol
in a 96-well plate.
Is the neutrophil reactive oxygen species production measured by luminol
and lucigenin chemiluminescence intra or extracellular?
There were Dustin Prince, a charming but lazy forensic investigator; Chance Luminol
, an egotistical crime lab director; Sarah Aupare, a quiet nursemaid; and a host of other secretive characters.
The third section addresses investigative tools: forensic anthropology and odontology, exhumation, criminal investigative analysis, wiretaps, bloodstain pattern analysis, luminol
, questioned documents, polygraphs, geographic profiling, and forensic art.
The slices were incubated in a buffered solution containing antirabbit antibody labeled with horse rabbit peroxidase and assayed for luminescence using a 1:1 solution of a dilute luminol
solution (0.1 MG %) and hydrogen peroxide (0.3%).