The cell culture assays were performed after transfection of (i) pBIND-PPAR[beta]/[delta], expressing Gal4-DBD and PPAR[beta]/[delta]-LBD fusion protein, and (ii) pGRE-LUC, which contains a Gal4 response element followed by firefly
luciferase reporter gene.
A different approach to imaging more than one cell population is to use
luciferases with different substrates, which allows the signal from each cell population to be acquired in sequence, as opposed to simultaneously, thus precluding the need for data acquisition with different emission filters and the postacquisition processing for spectral decomposition.
Luciferases are used to produce bioluminescence in order to visualize cellular activities and to track cell populations in vivo.
The kinetic ans structural properties of different species of firefly
luciferases have been found different due to alteration in their 3-dimentional structures.
Luciferases generating redder light have been developed (SN: 8/31/96, p.
The cloning and characterization of the novel
luciferases from these chaetognath species will be a fascinating glimpse into the evolution of coelenterazine-based bioluminescence.
ATP formed by action of the enzyme label on an acetyl phosphate substrate is measured using the firefly
luciferase reaction.
By linking various
luciferase genes to other genes of interest spliced into cells, scientists can now measure the relative activity rates of multiple genes in cells over time.
The purification of the solubilized
luciferases from 40 g of ovaries involved over 50 column chromatography runs (summarized in Table 1).
The activity of
luciferases in catalyzing light emission, i.e.
Cell Culture, Transient Transfection, and
Luciferase Assays.
Identification of Responsible
Luciferase Gene by Specific PCR.
