The number of a U3 repeat box acting as an enhancer in long terminal repeats of polytropic replication-competent porcine endogenous retroviruses dynamically fluctuates during serial virus passages in human cells.
Sequence analysis of porcine endogenous retrovirus long terminal repeats and identification of transcriptional regulatory regions.
Previous findings indicate that the regulatory region of PERV for retroviral transcription, replication and integration into the cellular DNA is located on the 5' Long Terminal Repeat (LTR).
The 5' Long Terminal Repeat (LTR) located in the promoter region has been shown to be influential in the transcription of replication-competent PERV (Scheef et al.
Short interspersed nuclear elements (Alu and MIR), a long terminal repeat
(MaLR), and long interspersed nuclear elements (L1 and CR1) were found within 1 kb of the BclI-relevant sites in BX842559, BX682237, and BX276110, and thus would restrict potential primer target sequences (data not shown).