After inoculating the Sporosarcina Pasteurii into the mixed culture medium for 4 hours, the concentration of ammonia nitrogen rose rapidly, which indicated that Sporosarcina Pasteurii had a high urease activity in the initial state, but the production rate of CaC[O.sub.3] increase slowly because of the bacteria are still in the lag phase or logarithmic phase
which have insufficient bacteria and urease.
The results in Figures S1 and S2 (supplementary data) show that CCCP does not affect the growth rate of A, baumannii 34702 (in logarithmic phase
: A, baumannii + Glu: 0.18 [h.sup.-1] and A, baumannii + Glu + CCCP: 0.33 [h.sup.-1]) or K, pneumoniae 28296 (in logarithmic phase
: K, pneumoniae + Glu: 0.19 [h.sup.-1] and K, pneumoniae + Glu + CCCP: 0.14 [h.sup.-1]).
The significant increase of cell density indicate that the growth is at the logarithmic phase
where the yeast is actively undergoing cell division.
The sigmoid growth pattern in all cases showed that cells started their logarithmic phase
of growth at 4th day of subculture and cells remained more active during that period.
Briefly, parasites were harvested from logarithmic phase
culture by centrifugation at 800g for 5 min and washed three times in PBS with Hoechst 33342 at 5 [micro]g/mL.
The growth curves for both bacteria were recorded in absence of metal cholates, and it's possible to note that the time for the logarithmic phase
were 25 and 30 minute for P.
The logarithmic phase
is followed by the stationary phase in which the number of organisms is stable.
cell-free filtrate contained an average of 7.4 X [10.sup.7] particles [ml.sup.-1] and the stationary phase cell-free filtrate contained 2.09 X [10.sup.8] particles [ml.sup.-1].
The isolated strain was characterized by logarithmic phase
in first 8h and stationary phase in the following 8-120 h.
Oleuropein and hydroxytyrosol exhibited the best inhibitory effect among the natural products tested in both stationary and middle logarithmic phase
promastigotes of L.
So, in marine broth culture, production of antibacterial compound with PG-01 isolate was started in the early stage of logarithmic phase
and increased to a maximal production level until the stationary phase; that it can be said that antibacterial production occurs during the whole growth phase.
Lag time before logarithmic phase
growth about 24h followed by an exponential phase leading to maximal cell number at day 7.