MCF-7 cells in the logarithmic growth phase
were harvested and randomly seeded into 6-well plates at an initial density of 5 x [10.sup.6] cells per well.
Logarithmic growth phase
(log phase) cell cultures of Roseivivax sp.
Cells in the logarithmic growth phase
were cultured at a density of 10,000 cells/well in 24-well plates.
Single-layer of cells grows against the wall; when they grow till 80% to 90%, cells are merging, trypsin enzyme-digesting cells are subcultured, and logarithmic growth phase
cells are collected for experiment.
In each passage, cells at the logarithmic growth phase
were collected and counted with a hemocytometer and then resuspended in frozen solution including 10% dimethyl sulfoxide (DMSO; MP Bio cat.
Vascular smooth muscle cells in logarithmic growth phase
were used and divided into eight groups: (1) control group (group C) where cells were continuously cultured in 10% FBS DMEM; (2) U-II group (group M) where [10.sup.-8] mol/L U-II was added; (3) positive control group (Flu group) where in the presence of [10.sup.-8] mol/L U-II and [10.sup.-7] mmol/L fluoxetine (Flu); (4) urantide-treated groups where in the presence of [10.sup.-8] mol/L U-II, [10.sup.-10], [10.sup.-9], [10.sup.-8], [10.sup.-7] and [10.sup.-6] mol/L of urantide were added, respectively.
A slow phase of smut pathogen growth was observed from 0 h to 12 h; then a logarithmic growth phase
appeared from 12 h to 60 h, followed by a stationary phase and declining phase.
Each bacterium was then removed at the end of logarithmic growth phase
, and was asepticcally transferred to plastic containers containing sterile perlite and was then mixed well.
No significant loss in the titre of adsorbed virus was detected in the insect cells and the logarithmic growth phase
of the virus started from 5 h PI.
C6 glioma cells in logarithmic growth phase
were treated with swainsonine at doses of 1.6, 0.4 and 0.1 [micro]g/ml and CDDP of 3 [micro]g/ml as the positive group.
Growth inhibition in vitro: To culture SGC-7901 cells in logarithmic growth phase
, we added swainsonine at different concentrations, as follows: 20, 10, 5, 2.5, 1.25, 0.625, 0.30, 0.15, 0.08 and 0.04 [micro]g/ml; we simultaneously established the blank group as zero (adding culture medium only), negative control group (inoculating SGC-7901 cell and adding culture medium only, no medication) and 5-Fu of 10 [micro]g/ml as the positive group, with eight parallel wells in each group.
We found that cells starved from logarithmic growth phase
give rise to an increase in cell concentration of 10-30%, whereas cells starved from late logarithmic growth phase
or stationary growth phase give rise to only a small, if any, increase in cell concentration.