The dependent variables evaluated were the average weight and total length growth, survival, digestive enzymatic activity (alkaline proteases, acid proteases, trypsin, chymotrypsin, and lipase), as well as the relative expression of lipogenic
genes: acetyl CoA carboxylase (ACC1) and fatty acid synthase (FAS).
Metabolic factors involved in lipogenesis (i.e., FAS, SCD-1, and ACC) were detected using Western blotting analysis, and gene expression of the lipogenic
pathway regulators SREBP-1c and ChREBP was evaluated using polymerase chain reaction (PCR).
Diets can alter the synthesis and deposition of FA in muscles which can modulate the expression of lipogenic
Evidence of genomic and non-genomic regulation of lipogenic
and oxidative pathways.
Beylot, "Increased hepatic lipogenesis but decreased expression of lipogenic
gene in adipose tissue in human obesity," American Journal of Physiology-Endocrinology and Metabolism, vol.
Ghrelin, originally identified as a growth hormone secretagogue with orexigenic and lipogenic
effects, has also been shown to play significant roles in glucose regulation.
extract by down-regulation of the expression of lipogenic
genes in high-fat diet-induced obese mice," Molecular Medicine Reports, vol.
In addition, PPAR[gamma] was demonstrated to play a crucial role in the maintenance of stemness in ErbB2-positive breast cancer cells; PPAR[gamma] antagonist GW9662 induces apoptosis and inhibits tumorsphere formation and tumor formation through the inhibition of lipogenic
genes (ACLY, MIG12, FASN, and NR1D1) and stem cell-related genes (KLF4 and ALDH) .
Lemoine et al., "The lipogenic
transcription factor ChREBP dissociates hepatic steatosis from insulin resistance in mice and humans," The Journal of Clinical Investigation, vol.
In addition, we identified an obvious downregulation of hepatic lipogenic
gene expression (Acc and Fas), while Srebp1c and genes involved in fatty acid oxidation (Cpt1a and Acox1) were unaltered (Figure 3(c)).
We defined abnormal lipids as elevated lipids (cholesterol, triglycerides, or fatty acids) in blood or liver, increased liver weight (as surrogate measurement of hepatic steatosis), and increased activity of liver lipogenic
Well-Differentiated Spindle Cell Liposarcoma (Atypical Spindle Cell Lipomatous Tumor).--This entity is a recently reported [CD34.sup.+] low-grade malignant lipogenic
neoplasm that shares some similar histologic and molecular features as SCL.