One day after lipofection
, GFP-expressing cells were sorted using a FACSAria III cell sorter (Becton, Dickinson and Co.
HSA-PEI/pEGFP had been shown to provide better pEGFP transfection efficiency than PEI/pEGFP, jetPEI/pEGFP or lipofection
Methods: Stably RIP140-overexpressing N2a (N2a-RIP140) cells were used as a neurodevelopmental model, and were constructed by lipofection
and overexpression validated by real-time polymerase chain reaction and Western blot.
Lipofectamine, according to the manufacturer's lipofection
Development of such a delivery system for therapeutic purposes faces many challenges, including ethical and technical issues; for example, choice of the best gene transfection method to introduce the RNA/protein of interest (cargo) into exosome-secreting cells (virus, lipofection
, electroporation), how to target the cargo into the exosomes, the best technique to load exosomes with the drug of interest (electroporation or lipofection
), and the cost of the process are a few of the concerns that need to be addressed.
4% was the highest transfection efficacy obtained using the lipofection
method (Figure 3).
The cell density for lipofection
was adjusted to 90% - 95%.
Cell analysis, the second largest life science instrumentation market, is made up of many subsegments, including electroporation and lipofection
, the largest of which is flow cytometry.
Fish and chicken embryos were also transfected using lipofection
, electroporation or high velocity microprojectiles (Szelei et al.
HeLa cells were transiently transfected using the lipofection
We employed some physical and chemical gene transfer methods (electroporation, Lipofection
and calcium phosphate) for SP2/0 transfection, but none was found to be efficient (data not presented).
2] fraction (B), EtOAC fraction (C), n-BuOH fraction (D), activated trascription of ER[alpha] or ER[alpha] in HeLa ER[beta] by lipofection