A mixture predominantly of phospholipids used for aiding in the transfer of DNA into cells.
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In order to improve transfection efficiency, we tested different ratios of ZFN plasmid to lipofectin 2000 ([micro]g/[micro]L): 2:4, 2:5, 2:6, 3:6, and found there was no distinct difference in transfection efficiency (data not shown).
Lipofectin 2000 was used to transfect MSCV-empty vector (Clontech, USA)-or MSCV-ShRNA plasmids-into HGC-27 gastric cancer cells according to manufacturer's instruction (Invitrogen, USA).
Plasmids were transiently transfected into the cells using Lipofectin Reagent (GibcoBRL).