A mixture predominantly of phospholipids used for aiding in the transfer of DNA into cells.
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For LOX-1 siRNA transfection, J774A.1 cells were transfected with100 nM LOX-1 siRNA using the lipofectin 2000 transfection reagent (Invitrogen, USA).
Lipofectin (Invitrogen, Carlsbad, CA) was used as a positive control for transfection.
Turkson, University of Central Florida, Orlando, FL) or pGASLuc plasmid by using Lipofectin reagent (Invitrogen), according to the manufacturer's instructions.
In order to improve transfection efficiency, we tested different ratios of ZFN plasmid to lipofectin 2000 ([micro]g/[micro]L): 2:4, 2:5, 2:6, 3:6, and found there was no distinct difference in transfection efficiency (data not shown).
Lipofectin 2000 was used to transfect MSCV-empty vector (Clontech, USA)-or MSCV-ShRNA plasmids-into HGC-27 gastric cancer cells according to manufacturer's instruction (Invitrogen, USA).
For RhoA siRNA, RAW 264.7 cells were transiently transfected with 10 nM RhoA-targeting siRNA (sense: 5'-GAA GUC AAG CAU UUC UGU CTT-3'; antisense: 5'-GAC AGA AAU GCU UGA CUU CTT3') premixed with 6 [micro]g/mL of Lipofectin (Invitrogen).
The authors were able to demonstrate efficient p53 protein expression compared to commercially available Lipofectin suggesting that SLNs could be used as highly efficient gene therapy vehicles in lung cancer.
The ternary complex of pDNA/siRNA/[alpha]-CDE showed higher siRNA sequence-specific gene silencing effects without off-target effects than those of commercial transfection reagents such as Lipofectamine2000 TransFast and Lipofectin in various cells [14,15].
Lipofectin: a highly efficient, lipid-mediated DNA transfection Procedure.
Plasmids were transiently transfected into the cells using Lipofectin Reagent (GibcoBRL).
LDH/GFP (Green fluorescence protein) gene promoter/reporter constructs were designed for in vitro transfection using polycationic liposomes (Lipofectin, Lipofectamine, DMRIE & Cellfectin) and the non-liposomal liquid, Effectene.