Resistance source References ([dagger]) PI 438489B Yue et al., 2001a A2# B1 C1 C2 Dla E G# PI 90763 Guo et al., unpublished A2 Ill E G J L Concibido et al., 1997 G J PI 40198A Guo et al., unpublished A2 BI G N PI 467312 ([dagger][dagger]) Lu et al., unpublished Cl G E J Peking Qiu et al., 1999 B2 Meksem et al., 2001 A2 G Concibido et al., 1997 G N Mahalinggam and Skorupska, 1995 A2 P1209332 Concibido et al., 1994 A2 G J Concibido et al., 1996 D2 G PI89772 Yue et al., 2001b BI D2# E# G P188788 Glover et al., 2004 G J Concibido et aL,1997 G PI437654 Webb et al., 1995 A2 G M Hartwig ([double dagger] [double dagger]) Schuster et al., 2001 D2 Vierling et al., 1996 B1# J87-233 ([subsection]) Heer et al., 1998 A2 G P1468916 (G.
Whereas many loci may contribute to the physiological differentiation between the taxa, a preponderance of the taxon-specific variation is encoded by genes within the linkage group
marked by the Est locus.
Sixteen male linkage groups
were mapped with an average number of 6.8 loci per linkage group
, and each linkage group
had 2-15 markers (Table 2).
Despite its apparent ubiquity in eukaryotes, the evolutionary significance of and the mechanisms that promote linkage group
conservation have yet to be identified.
The allele sizes of amplified products were reviewed by two different researchers, compiled in an excel sheet, checked for potential genotyping errors, and transferred to a CRIMAP software format for linkage analysis using limits-of-detection (LOD) scores of 3.0 and 5.0 to identify linkage groups
(LG) and determine marker order.
Using CIM, we identified 40 QTL for eight seed traits in 14 marker intervals on 10 linkage groups
U = unlinked markers with LOD score of 3.0 or 5.0: TUMXLv5.42, 5.66, 6.124, 7.9, 7.32, 8.48, 8.75, 8.101, 8.184, 8.191, 8.193, 8.206, 8.216, 8.224, 9.60, 9.77, 9.78, 9.103, 9.180, 10.204, 10.238, 10.278, 10.284, H16.1, GW7-9.226, GWS-7-284.2, GW3-5.271, gw7-9.94, SU352, SU508, TSV2A003.H2, TSV2A002.F8, TSV2A003.F2, TSV2A003.A4, D28118, 026403 U * = unlinked, refers to some of the 29 markers that located on to linkage group
4 using CRIMAP with LOD score of 3.0, but were unable to order them.
Marker pairs were tested only for the 465 pairwise combinations of the 31 linkage groups
because markers within linkage groups
Eighty-five TRAP markers were positioned across all 11 linkage groups
A nine-marker 50-cM region of linkage group
C2 was analyzed (Fig.
Markers generated by any primer combination that were clustered on only one linkage group
or in only one region were not found.
Abbreviations: LOD, log-likelihood; MLG, molecular linkage group
; PCR, polymerase chain reaction; PI, plant introduction; QTL, quantitative trait loci; RGA, resistance gene analog: RFLR restriction fragment length polymorphism: SSR, simple sequence repeat.