ligase chain reaction


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ligase

 [li´gās, lig´ās]
any of a class of enzymes that catalyze the joining together of two molecules coupled with the breakdown of a pyrophosphate bond in ATP or a similar triphosphate.
DNA ligase either of two enzymes that join two double-helical molecules of DNA together to make a longer DNA molecule.
ligase chain reaction a type of DNA amplification that uses DNA ligase to link chains and amplify the template containing the sequence in question.

ligase chain reaction

a technique for target amplification of DNA in which DNA ligase is used to join two complementary oligonucleotide probes that have bound to a target sequence in vitro. The ligation product is used as a template for ligation of complementary oligonucleotides that, through repeated enzymatic processing, allow for logarithmic accumulation of products that can be used to determine the presence of the target of interest.

ligase chain reaction

a type of DNA amplification that uses DNA ligase to link chains and amplify the template containing the sequence in question.

ligase chain reaction

Ligation amplification reaction Molecular biology A DNA amplification technique for detecting minimal amounts of a known DNA sequence, similar in principle to PCR. See PCR.

li·gase chain re·ac·tion

(lī'gās chān rē-ak'shŭn)
A technique for target amplification of DNA in which DNA ligase is used to join two complementary oligonucleotide probes that have bound to a target sequence in vitro. The ligation product is used as a template for ligation of complementary oligonucleotides that, through repeated enzymatic processing, allow for logarithmic accumulation of products that can be used to determine the presence of the target of interest.

ligase

an enzyme that repairs single-strand nicks in duplex DNA and covalently joins DNA fragments with complementary, overlapping (called also cohesive or sticky) ends or less efficiently, with blunt ends. Bacteriophage T4 ligase catalyzes the formation of a covalent phosphodiester bond between adjacent 5′-phosphate and 3′-hydroxyl groups in duplex DNA.

ligase chain reaction
a technique for detecting a specific nucleotide pair in a gene. Called also LCR.
References in periodicals archive ?
Evaluation of the Abbott LCx ligase chain reaction assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine and genital swab specimens from a sexually transmitted disease clinic population.
Prospective comparison of the Gen-Probe PACE 2 assay and the Abbott ligase chain reaction for the direct detection of Chlamydia trachomatis in a low prevalence population.
Detection of Chlamydia trachomatis endocervical infections by ligase chain reaction versus ACCESS Chlamydia antigen assay.
Preliminary evaluation of the ligase chain reaction for specific detection of Neisseria gonorrhoeae.
Detection of Chlamydia trachomatis by ligase chain reaction compared with polymerase chain reaction and cell culture in urogenital specimens.
A small sample was then removed from the remaining fluid for testing with the Ligase Chain Reaction (LCR) Chlamydia assay, a sensitive amplified DNA method.
Ligase chain reaction to detect Chlamydia trachomatis infection of the cervix.
Analysis of ligase chain reaction products amplified in a silicon-glass chip using capillary electrophoresis.
The ligase chain reaction has also been described as an alternative to dot-blot analysis and allele-specific PCR [18].
Ligase chain reaction (LCR) and other ligation-based assays such as gap LCR and the oligonucleotide ligation assay have been used independently or coupled to the polymerase chain reaction to diagnose genetic and infectious diseases [1, 2].