ligase chain reaction


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ligase

 [li´gās, lig´ās]
any of a class of enzymes that catalyze the joining together of two molecules coupled with the breakdown of a pyrophosphate bond in ATP or a similar triphosphate.
DNA ligase either of two enzymes that join two double-helical molecules of DNA together to make a longer DNA molecule.
ligase chain reaction a type of DNA amplification that uses DNA ligase to link chains and amplify the template containing the sequence in question.

ligase chain reaction

a technique for target amplification of DNA in which DNA ligase is used to join two complementary oligonucleotide probes that have bound to a target sequence in vitro. The ligation product is used as a template for ligation of complementary oligonucleotides that, through repeated enzymatic processing, allow for logarithmic accumulation of products that can be used to determine the presence of the target of interest.

ligase chain reaction

Ligation amplification reaction Molecular biology A DNA amplification technique for detecting minimal amounts of a known DNA sequence, similar in principle to PCR. See PCR.

li·gase chain re·ac·tion

(lī'gās chān rē-ak'shŭn)
A technique for target amplification of DNA in which DNA ligase is used to join two complementary oligonucleotide probes that have bound to a target sequence in vitro. The ligation product is used as a template for ligation of complementary oligonucleotides that, through repeated enzymatic processing, allow for logarithmic accumulation of products that can be used to determine the presence of the target of interest.
References in periodicals archive ?
Detection of Chlamydia trachomatis endocervical infections by ligase chain reaction versus ACCESS Chlamydia antigen assay.
Performance of transcription-mediated amplification and ligase chain reaction assays for detection of chlamydial infection in urogenital samples obtained by invasive and noninvasive methods.
Preliminary evaluation of the ligase chain reaction for specific detection of Neisseria gonorrhoeae.
These methodologies include allele-specific hybridization (27), allele-specific amplification (28), ligase chain reaction (29), and designed restriction fragment length polymorphism (7).
Detection of Chlamydia trachomatis by ligase chain reaction compared with polymerase chain reaction and cell culture in urogenital specimens.
Ligase chain reaction to detect Chlamydia trachomatis infection of the cervix.
Diagnosis of Chlamydia trachomatis urethral infection in symptomatic and asymptomatic men by testing first-void urine in a ligase chain reaction assay.
Analysis of ligase chain reaction products amplified in a silicon-glass chip using capillary electrophoresis.
The ligase chain reaction has also been described as an alternative to dot-blot analysis and allele-specific PCR [18].
Ligase chain reaction (LCR) and other ligation-based assays such as gap LCR and the oligonucleotide ligation assay have been used independently or coupled to the polymerase chain reaction to diagnose genetic and infectious diseases [1, 2].
(4) Nonstandard abbreviations: LCR, ligase chain reaction; hGH, human growth hormone; and 1x SSC, 150 mmol/L NaCl and 15 mmol/L sodium citrate, pH 7.