ligase


Also found in: Dictionary, Acronyms, Encyclopedia, Wikipedia.

ligase

 [li´gās, lig´ās]
any of a class of enzymes that catalyze the joining together of two molecules coupled with the breakdown of a pyrophosphate bond in ATP or a similar triphosphate.
DNA ligase either of two enzymes that join two double-helical molecules of DNA together to make a longer DNA molecule.
ligase chain reaction a type of DNA amplification that uses DNA ligase to link chains and amplify the template containing the sequence in question.

li·gase

(lī'gās),
Generic term for enzymes (EC class 6) catalyzing the joining of two molecules coupled with the breakdown of a pyrophosphate bond in ATP or a similar compound.
See also: synthetase.

ligase

/li·gase/ (li´gās) (lig´ās) any of a class of enzymes that catalyze the joining together of two molecules coupled with the breakdown of a pyrophosphate bond in ATP or a similar triphosphate.

ligase

(lī′gās′, -gāz′)
n.
Any of a class of enzymes that catalyze the linkage of two large molecules.

li·gase

(lī'gās)
Generic term for enzymes (EC class 6) catalyzing the joining of two molecules coupled with the breakdown of a pyrophosphate bond in adenosine triphosphate or a similar compound.
See also: synthetase

ligase

An ENZYME that promotes the linkage of chemical groups. Also known as a synthetase.

ligase

an enzyme that catalyses the joining of short molecules into longer ones, for example, DNA LIGASE.

ligase

an enzyme that repairs single-strand nicks in duplex DNA and covalently joins DNA fragments with complementary, overlapping (called also cohesive or sticky) ends or less efficiently, with blunt ends. Bacteriophage T4 ligase catalyzes the formation of a covalent phosphodiester bond between adjacent 5′-phosphate and 3′-hydroxyl groups in duplex DNA.

ligase chain reaction
a technique for detecting a specific nucleotide pair in a gene. Called also LCR.
References in periodicals archive ?
Oncogenic role of the E3 ubiquitin ligase NEDD4-1, a PTEN negative regulator, in non-small-cell lung carcinomas.
Based on ligand-inducible degradation of ER[alpha], (14,15,42) degradation of ER[alpha] by Hbo1, (31) and inhibition of E3 ligase activity of Hbo1 for ER[alpha] by the ligand (Fig.
Chen CNJ, Brown-Borg HM, Rakoczy SG, Ferrington DA, Thompson LV Aging impairs the expression of the catalytic subunit of glutamate cysteine ligase in soleus muscle under stress.
Similarly, the ubiquitin ligase HRD1, which normally promotes APP ubiquitinylation and degradation resulting in decreased generation of A[beta], was found impaired in AD brain [59].
In particular, the reliance on Ligase 3 appears, in turn, to be dependent upon the activity of another key DNA repair gene, p53.
The reaction proceeds by repeatedly cycling the reaction temperature between denaturation (95[degrees]-99[degrees]C) and a lower temperature suitable for sequence specific annealing and DNA ligase activity (depending on sequence composition and length of the primers, but generally in the 50[degrees]-60[degrees]C range).
MARCH5 autoubiquitinates itself and its E3 ubiquitin ligase activity is required to prevent accumulation of fragmented mitochondria [38].
This cloning method allows transferring of DNA fragment into different cloning vectors without using restriction endonucleases and ligase.
METHODS: In male Sprague-Dawley rats (n = 8/group; 10d juice intervention, 4d wash-out; 4 treatment cycles), expression of target genes (superoxide dismutase, SOD1/SOD2; glutathione peroxidase, GPX1/GPX2; [gamma]-glutamylcysteine ligase, GCLC/GCLM; glutathione reductase, GSR; catalase, CAT; NAD(P) H:quinone oxidoreductase-1, NQO1 and transcription factor erythroid-derived 2-like-2, Nrf2) was quantified with duplex RT-PCR, using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as control.
These include some genotypic methods like the DNA probes, Nucleic acid amplification, Polymerase chain reaction and the Ligase chain reaction and the phenotypic methods like the FAST Plaque TB.
WSB1 on chromosome 17 is a hedgehog-inducible ubiquitin ligase, and its loss results in spontaneous intestinal inflammation.