Ligand-gated ion channels typically consist of an extracellular domain that includes the ligand-binding site
and a transmembrane domain that includes the ion pore.
GSTs consist of a specific-glutathione (GSH)-binding site (G-site) next to a non-specific electrophilic ligand-binding site
Interactions between these odorants and receptors in the ligand-binding site
will be modelled by studying complexes formed between odorants and mimics of amino acid residues.
and can be inhibited by phosphocholine, indicating that the single promiscuous ligand-binding site
on the B face can accommodate diverse structural groups.
METHODS: We performed computational modeling to determine interaction energies between the ligand and the AR ligand-binding site
, and measured in vitro competitive binding assays for AR by polarization fluorometry analysis.
Lipocalins have a conserved folding pattern consisting of antiparallel [beta]-strands that form a [beta]-barrel with a distinct ligand-binding site
This type of response would be expected if hER possesses multiple ligand-binding sites
displaying cooperative behavior as has been reported (14,16).