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Standard leukopheresis processing of 14 L of blood from the donor was performed without problems.
Sipuleucel-T consists of autologous antigen presenting cells enriched for a CD54+ DC fraction that are harvested by leukopheresis and cultured with a fusion protein (PA2024), comprising of prostate acid phosphatase (PAP) and granulocyte-monocyte colony stimulating factor (GMCSF) [47, 48].
Patients undergo leukopheresis with CD4-cell isolation.
Available instruments can be calibrated to perform plasmapheresis, plateletpheresis (collection of donor platelets for patient use), erythrocytopheresis (used for treatment of sickle cell anemia), or leukopheresis (collection of donor stem cells for transplantation; removal of white blood cells for therapeutic purposes).