cell suspensions representing 20 serovars were added to serially diluted serum specimens in 96 wells microtiter plates and were incubated at 30degC for 2 hours.
penetrate the host for lesions on the skin and mucous membranes.
The primary aim was to identify factors that exposed them to a leptospire
contaminated environment including farming activities, irrigation practices and animal rearing.
A final concentration of 1.5 x [10.sup.11] leptospires
[ml.sup.-1] were obtained, as determined by direct cell counting in a Petroff Hausser chamber.
These factors could increase the leptospire
count in contaminated water.
Eighty seven percent were positive for PCV2 (87/100), 68% were positive for PPV (68/100), and 22% were positive for pathogenic leptospires
Isolation is difficult and not always successful, and the detection of leptospires
in body fluids using dark-field microscopy is limited owing to proteinaceous filaments (pseudoleptospires) (11).
Leptospirosis is a bacterial infection caused by leptospires
or spirochetes, usually transmitted when one is exposed to rat urine and feces.
Efficient detection of pathogenic leptospires
using 16S ribosomal RNA.
In the study period, mean annual maximum temperature ranged from 32.1 to 37.8[degrees]C, which hinders the survival of leptospires
in the environment.