1959) The relation between the activity of a lecithinase
and the electrophoretic charge of the substrate.
7, gelatin hydrolysis, indole production, citrate utilization, tyrosine utilization, VP-pH, VP- Acetoin, arabinose utilization, mannitol utilization, xylose utilization, methyl red-voges proskauer (MR-VP), oxidase production, catalase production, starch hydrolysis, nitrate reduction, casein hydrolysis, gas production from glucose, lecithinase
production and production of SO2) according to the Bergey's Manual of Determinative Bacteriology (Bergey and Holt, 1994)
All isolates were phenotypically identical; they produced broad halos of hemolysis and lecithinase
reactions and had positive glucose-1-phosphate acidification test results, reflecting the constitutive activation of the PrfA virulence regulon.
perfringens (A, B, C, D and E) and exhibits lecithinase
activity in addition to its lethal and necrotic activities (Murray et al.
The isolates were identified based on the litmus milk test, gelatinase, deoxyribonuclease (DNase), lecithinase
and fermentation of glucose and lactose (Holt et al.
pseudomallei produce toxins such as pyocyanin (blue-green pigment that interferes with energy production), lecithinase
(causes cell lysis), collagenase, lipase, and hemolysin.
OCLA detects B-glucosidase activity, common to all Listeria species, and the lecithinase
activity of L.
Both strains were found have the ability to produce amylase, protease, cellulase, and pectinase enzymes; whilst they couldn't be produced lecithinase
Biochemical reactions, sugar fermentation tests, bile solubility, starch hydrolysis, gelatin digestion, catalase, lecithinase
, indole production and nitrate reduction test, which are the most characteristics tests to differentiate between them .
This isolate also produced extracellular enzymes such as lecithinase
, xylanase and amylase.
ivanovii, both of which have the ability to produce the phospholipase enzyme, lecithinase
Tests for catalase, indole, lecithinase
, lipase, and reverse Christie-Atkins-Munch-Peterson were negative.