Chang et al., "Epstein-barr virus latent membrane protein 2A promotes invasion of nasopharyngeal carcinoma cells through ERK/Fra-1-mediated induction of matrix metalloproteinase 9," Journal of Virology, vol.
Longnecker, "Latent Membrane Protein 2A Inhibits Transforming Growth Factor-[beta]1-Induced Apoptosis through the Phosphatidylinositol 3-Kinase/Akt Pathway," Journal of Virology, vol.
EBVaGC belongs to latency type I, where the viral genes EBV nuclear antigen 1 (EBNA1), latent membrane protein 2A (LMP2A), Bam HI-A rightward transcripts (BARTs), and EBV-encoded small RNA (EBER1/2) may be expressed [10, 38, 39].
Inoue et al., "Survival advantage of EBV-associated gastric carcinoma: survivin up-regulation by viral latent membrane protein 2A," Cancer Research, vol.
Bornkamm, "Control of Epstein-Barr virus reactivation by activated CD40 and viral latent membrane protein 1," Proceedings of the National Academy of Sciences of the United States of America, vol.
Fujiwara et al., "Detection of Epstein-Barr virus-encoded small RNA 1 and latent membrane protein 1 in synovial lining cells from rheumatoid arthritis patients," International Immunology, vol.
EBV latent membrane protein
1 (LMP-1) protects infected B cells from apoptosis by up regulation of the bcl-2.
Induction of bcl-2 expression by Epstein Barr virus latent membrane protein
1 protects infected B cells from programming cell death.
Immunodetection for Epstein-Barr virus was negative with immunohistochemistry (latent membrane protein
1) and in situ hybridization (Epstein-Barr virus-encoded RNA 1).
Immunohistochemistry, after microwave antigen retrieval using the streptavidin-biotin complex technique and diaminobenzidine as the chromogen, was performed on the formalin-fixed, paraffin-embedded tissue with the following antibodies: EBV latent membrane protein 1 (CS1-4; dilution 1:65; Dakopatts, Glostrup, Denmark); CD20 (1:100; Dakopatts); CD79a (1:100; Dakopatts); CD3 (1:75; Dakopatts); CD15 (1:50; Dakopatts); CD30 (1:20; Dakopatts); CD68 (1:50; Dakopatts), AE1/3 (1: 1600; monoclonal; Signet Laboratories, Dedham, Mass); CAM 5.2 (1:400; monoclonal; Becton Dickinson, San Jose, Calif); epithelial membrane antigen (1:65; monoclonal; Dakopatts), and S100 protein (1:1200; polyclonal; Signet).
The large tumor cells were negative for all other markers, including EBV latent membrane protein 1.
Immunohistochemical staining for EBV latent membrane protein
(Dako) was negative.