EBVaGC belongs to latency type I, where the viral genes EBV nuclear antigen 1 (EBNA1), latent membrane protein 2A (LMP2A), Bam HI-A rightward transcripts (BARTs), and EBV-encoded small RNA (EBER1/2) may be expressed [10, 38, 39].
Survival advantage of EBV-associated gastric carcinoma: survivin up-regulation by viral latent membrane protein 2A," Cancer Research, vol.
Longnecker, "Epstein-Barr virus latent membrane protein 2A mediates transformation through constitutive activation of the Ras/PI3-K/Akt pathway," Journal of Virology, vol.
EBV latent membrane protein
1 (LMP-1) protects infected B cells from apoptosis by up regulation of the bcl-2.
Induction of bcl-2 expression by Epstein Barr virus latent membrane protein
1 protects infected B cells from programming cell death.
Immunohistochemistry, after microwave antigen retrieval using the streptavidin-biotin complex technique and diaminobenzidine as the chromogen, was performed on the formalin-fixed, paraffin-embedded tissue with the following antibodies: EBV latent membrane protein 1 (CS1-4; dilution 1:65; Dakopatts, Glostrup, Denmark); CD20 (1:100; Dakopatts); CD79a (1:100; Dakopatts); CD3 (1:75; Dakopatts); CD15 (1:50; Dakopatts); CD30 (1:20; Dakopatts); CD68 (1:50; Dakopatts), AE1/3 (1: 1600; monoclonal; Signet Laboratories, Dedham, Mass); CAM 5.
The large tumor cells were negative for all other markers, including EBV latent membrane protein 1.
Immunohistochemical staining for EBV latent membrane protein (Dako) was negative.
Furthermore, immunohistochemical staining for EBV latent membrane protein that was performed in the liver and lymph node was negative.