But the laser microdissection
system introduced at our unit at the start of the year is even more sophisticated than theirs.
We excised tissue areas corresponding to ~3000 to 5000 cells per probe by use of laser microdissection
and pressure catapulting microscope.
coupled with SELDI-TOF MS can overcome these limitations (18), but it is a time-consuming and labor-intensive procedure.
They present 15 contributions that describe a representative group of microarray laboratory methodologies, including noncontact laser microdissection
and pressure catapulting, sensitive detection of SARS coronavirus RNA by asymmetric multiplex nested RT-PCR amplification coupled with oligonucleotide microarray hybridization, molecular diagnostic testing for thrombophilia, protein microarray-based screening of antibody specificity, and multiplexed cytokine sandwich immunoassays.
Some of these individualized techniques include: fluorescence imaging and chemiluminescence imaging, both offered by Molecular Devices; gel and blot imaging from Amersham; fluorescence correlation spectroscopy from Zeiss; laser microdissection
from Leica Microsystems; and multi-well imaging from Atto.
Under the agreement, Leica will use Qiagen products and technologies in its laser microdissection
systems for collecting homogeneous populations of intact cells from solid tissue sections.
The chapter on noncontact laser microdissection
and pressure catapulting offers a detailed look at the use of laser capture microdissection for obtaining pure populations of cells for genomic analysis.
Assessment of RNA quality control has been improved by the use of highly sensitive gel analysis (3), and flow cytometry and laser microdissection
techniques allow isolation of tumor cells from nondiseased connective tissue as well as inflammatory infiltrates (4,5).