However, whether Cardiac glycoside Lanatoside C can induce oxidative stress in liver cancer cells and induce cell death both in vitro and in vivo remains unknown.
Results: In this study, we found that Cardiac glycosides, particularly Lanatoside C from Digitalis ferruginea could significantly inhibit PTEN protein adequate Huh7 and PTEN deficient Mahlavu human liver cancer cell proliferation by the induction of apoptosis and G2/M arrest in the cells.
Conclusion: The results indicated that Lanatoside C could be contemplated in liver cancer therapeutics, particularly in PTEN deficient tumors.
For this purpose, initially the cytotoxic activities of Lanatoside C along with other glycosides were tested on liver cancer cells.
Initial and majority of in vitro experiments were done with pure Lanatoside C (D.
Then 2 [micro]M Lanatoside C (L2261, Sigma Aldrich) was applied to the cells.
Control group mice (5 mice) received only simple syrup and other subjects received 6 mg/kg Lanatoside C (8 mice) in simple syrup by gavage feeding (5 days/week).
The cardiac glycosides Lanatoside A, Lanatoside C and Glucogitoroside showed significant levels of cytotoxicity whereas the phenylpropanoid glycosides had no notable effects on cell growth (Fig.
An increase in the G2/M phase was observed in cells treated with Lanatoside A, Lanatoside C and Glucogitoroside compared to cells in the DMSO controls (Fig.
Thus in this study, oxidative stress induction by Lanatoside C was monitored in HCC cells.
Based on the finding that Lanatoside C caused ROS accumulation, targets of ROS at the protein level was further investigated by western blot analyses.
In vivo anti-tumor effects of Lanatoside C on Mahlavu mice xenograft models