Lamin B1 is required for mouse development and nuclear integrity.
Lamin B1 levels modulate differentiation into neurons during embryonic corticogenesis.
miR-23 regulation of lamin B1 is crucial for oligodendrocyte development and myelination.
The primary antibodies for Bax, Bcl-2, GAPDH, and Lamin B1 were obtained from Proteintech (Wuhan, China).
After blocking with 5% nonfat milk, the membranes were incubated with Bax (1:500), Bcl-2 (1:500), cleaved caspase-3 (1:1000), cleaved PARP (1: 1000), p65 (1: 1000), GAPDH (1: 1000), and Lamin B1 (1: 500) antibodies overnight at 4[degrees]C.
These include the nucleus (tagged by the protein lamin B1
), mitochondria (Tom20), microtubules (alpha-tubulin), cell-to-cell junctions (desmoplakin) and adhesion (paxillin).
[beta]-Asarone appeared to increase expression of lamin B1. p53.
Lamin B1 is depressed in primary human and murine cell lines when they undergo senescence after DNA damage, replicative exhaustion, or oncogene expression (Shimi et al.
Consistent with the increased gene expression of these apoptosis-inducing factors (Figure 2(a)), cleavage of the caspase substrate Lamin B1 [36-40] was also increased by doxorubicin treatment in a substrate rigidity-dependent manner (Figure 2(b)).
(b) Total cell lysates were subjected to immunoblot analysis with antibodies against p21, Lamin B1, and [alpha]-tubulin as a loading control.