When the cell sheet encountered a non-adherent circle, lamellipodial
protrusions crawl around the circle, pulling the cells forward on the adhesive substrate.
Weeks et al., "Arp2/3 complex inhibition radically alters lamellipodial
actin architecture, suspended cell shape, and the cell spreading process," Molecular Biology of the Cell, vol.
analysis, cells were fixed with 3.7% formaldehyde and permeabilized with 0.1% Triton X, followed by staining with rhodamine phalloidin (Molecular Probes, Eugene, OR) according to the manufacturer's protocol and then images were acquired using fluorescence microscopy .
Heishi et al., "Impairment of VEGFA-stimulated lamellipodial
extensions and motility of vascular endothelial cells by chondromodulin-I, a cartilage-derived angiogenesis inhibitor," Experimental Cell Research, vol.
Time-Lapse Microscopy of Endothelial Cells and Analysis of Lamellipodial
Because both FAK and paxillin are the important regulators of lamellipodial
dynamics in motile cells , the formation of lamellipodium at the wound margin was investigated by immunostain with anti-phosphorylated-FAK and phosphorylated-paxillin antibody.
Self-assembly of actin cytoskeleton for polarity generation has been reported in physically pushed lamellipodial
fragments, in which the physical stimulus leads to local compression of the bundled actin filaments with myosin II, resulting in a positive feedback loop that initiates polarization and persistent migration .
Regulation of lamellipodial
persistence, adhesion turnover, and motility in macrophages by focal adhesion kinase.
Coelomocytes can be transformed from a lamellipodial
to a filopodial state by hypotonic shock, and this treatment can result in the cessation of retrograde flow.
If the actin cytoskeleton is firmly linked to the adhesion substrate through integrin, the lamellipodial
membrane will protrude; if the link is disconnected or slips, the reaction force will push back the actin network, resulting in the retrograde flow.
2011) whereas PDCD6IP was found to be critical in the formation of F-actin, stress fibres and lamellipodial
membrane protrusions (Pan et al.
Karniguian, "RhoA induces MMP-9 expression at CD44 lamellipodial
focal complexes and promotes HMEC-1 cell invasion," Experimental Cell Research, vol.