Lactophenol Cotton Blue stain
was used (Sigma-Aldrich, Germany) to study the microscopic characteristics of fungi, a drop of lactophenol solution was placed onto a clean slide.
A piece of colony was placed in a clean glass slide and added a drop of Lactophenol cotton blue stain
. After teasing the colony using a tooth pick it was viewed under high power of microscope.
During the first lab session, the students mount leaf squares in a few drops of lactophenol cotton blue stain
(20 mL phenol [Sigma-Aldrich, no.
The knobby appearance of macroconidia and microconidia indicated by Lactophenol Cotton Blue Stain
(Hardy Diagnostics, Santa Maria, CA, USA) of the mold form from fungal cultures is characteristic of H.
Leishman stain and lactophenol cotton blue stain
was used to identify the fungi.
The smears were examined after staining with Gram's stain/ Giemsa stain/ Lactophenol cotton blue stain
Additionally hair plucking and skin scraping were collected from 2-3 places and subjected to direct microscopic examination by staining with lactophenol cotton blue stain
. Woods lamp and direct microscopic positive samples were inoculated on Sabourauds Dextrose Agar (SDA) and Dermatophyte Testing Media (DTM) plates and incubated at 250C for 5-7 days with daily monitoring for presence of growth.
For microscopic observations, the fungal culture was stained as wet mount preparation with lactophenol cotton blue stain
. Final identification of fungal agents was made on the basis of colony characteristics and staining reaction (Larone 1993) observed under the microscope.
Gram, Ziehl-Neelsen and lactophenol cotton blue stains
were done for bacteria, mycobacteria and fungi respectively.