We considered including calcein acetoxymethyl ester (calcein AM), calcein acetoxymethyl ester violet (calcein violet), carboxyfluorescein succinimidyl ester (CFDA-SE, here and commonly referred to as CFSE), 4-(2-[6-(dioctylamino)-2-naphthalenyl]ethenyl)-1(3-sulfopropyl)pyridinium (di-8-ANEPPS), annexin V, lactadherin
, and Paul Karl Horan 67 (PKH67) (8, 9, 11-15).
The third way is to fuse the exogenous proteins with lipid-binding C1C2 domains of the human lactadherin
protein (MFGE8) noncovalently [85, 87, 88] (Figure 2(a)).
(c) MVs were defined as phosphatidylserine expressing (PS+) particles based on their ability to bind lactadherin
within the 0.1-1.0 [micro]m gate.
Tait, "Transmembrane voltage regulates binding of annexin V and lactadherin
to cells with exposed phosphatidylserine," BMC Biochemistry, vol.
Evidence of a role for lactadherin
in Alzheimer's disease.
binds to elastin--a starting point for medin amyloid formation?
It now appears that a constituent of breast milk, lactadherin
, has an anti-rotavirus activity.
The compound, called lactadherin
, is manufactured in the breast and doesn't break down in the baby's stomach.
Opsonins include antibodies, complement, fibronectin, mannose-binding lectin, and milk fat globulin (lactadherin
Subsequently, MVs were labeled by adding 10 [micro]L fluorescein isothiocyanate(FITC-) conjugated Lactadherin
(83 [micro]g [mL.sup.-1], Haematologic Technologies Inc., Vermont, USA) [29, 30], characterized by a phosphatidylserine- (PS-) bonding motif [29, 30].
Gluc is a reporter protein that emits bioluminescence when its substrate, coelenterazine, is present, and lactadherin
is a membrane-associated protein mainly found in exosomes.
Cai et al., "Induction of lactadherin
mediates the apoptosis of endothelial cells in response to advanced glycation end products and protective effects of grape seed procyanidin B2 and resveratrol," Apoptosis, vol.