In a large cohort including more than 350 myeloma patients, who participated in the Eastern Cooperative Oncology Group phase III clinical trial E9486, t(11;14)(q13;q32) was detected in approximately one-sixth of patients, and it was associated with a low serum monoclonal protein and plasma cell labeling index
and is less likely to be hyperdiploid by DNA content analysis, which appeared to correlate with a better survival and prognosis in those patients .
If all three criteria were met(morphologically suspicious lesion, p53 positivity, and high-Ki-67 labeling index
), tubal lesion is diagnosed as STIC, and if two criteria out of three are present it is diagnosed as serous tubal intraepithelial lesion(STIL).
The slides were prepared and were coated with K.2 gel emulsion (Ilford) prepared with distilled water at 40[degrees]C to determine thymidine labeling index
. After 3 days exposure at 4[degrees]C, autoradiograms were washed with D-19 b developer (Kodak) and fixed with Fixaj B (Kodak).
At this early interval, the labeling index
for the whole epithelium was 0.61, and more than 93% of the labeled cells were found in the basal stratum.
Several studies have emphasized the association of PCNA-LI (Proliferating Cell Nuclear Antigen Labeling Index
) with tumor malignancy grade and to have prognostic significance in a number of malignancies.
For women in the MPA group, the mean Ki-67 labeling index
decreased from 51.8% to 13.1% (P = .001).
BrdUrd-labelled cells were identified by immunohistochemistry, in which mouse anti-BrdUrd antibody (Santa Cruz biotechnology) was used, and enterocyte proliferation labeling index
also employed the method described by Iji et al.
The mean obtained was then used to calculate the labeling index
based on the ratio of the number of immunopositive cells per 1000 randomly counted cells per case studied, multiplied by 100 to express the index in percentage.
PCNA labeling index
was issued for determining the proliferative activity.
The aim of this study was to quantitate the differences between basal and luminal cells of PIN in relation to mean nuclear volume ([v.sub.V]nuc), and proliferating cell nuclear antigen labeling index
([LI.sub.PCNA]), and to compare these estimates with those obtained in normal prostate and carcinoma.
Examples of topics covered include the plasma cell labeling index
, multicolor spectral karyotyping, detection of chromosome 13 deletions by fluorescent in situ hybridization, identification and of clonotypic IgH VDJ sequences, real-time polymerase chain reaction of immunoglobulin rearrangements for quantitative evaluation of minimal residual disease, determination of telomerase activity and telomere length, clonality detection of the expanded t-cell populations in patients with multiple myeloma, and use of DNA micrrarray data.
Rapid clearance from serum, high labeling index
and absence of tissue specificity make Penetratin peptides efficient vehicles for systemic delivery of therapeutics interacting with drug targets in the cellular cytosol and nucleus.