knockout mouse


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Related to knockout mouse: Gene targeting

mouse

 [mows]
a small rodent, various species of which are used in laboratory experiments.
1. a small loose body.
2. a computer pointing device.
joint mouse a movable fragment of synovial membrane, cartilage, or other body within a joint; usually associated with degenerative osteoarthritis and osteochondritis dissecans.
knockout mouse a mouse that has had a specific gene artificially deleted from its genome.
nude mouse a mouse homozygous for the nu gene; these mice are hairless, lack a thymus, and thus lack T lymphocytes.
peritoneal mouse a free body in the peritoneal cavity, probably a small detached mass or omentum, sometimes visible radiographically.
SCID mouse (severe combined immunodeficiency) a strain of mice lacking in T and B lymphocytes and immunoglobulins, either from inbreeding with an autosomal recessive trait or from genetic engineering, used as a model for studies of the immune system.

knockout mouse

a mouse from whose genome a single gene has been artificially deleted.

Experimental animals lacking specific genes have become valuable research tools in many branches of medicine, including genetics, physiology, pharmacology, immunology, cell biology, and oncology. A transgenic animal is one into whose genome a foreign gene, constructed by recombinant DNA technology, has been deliberately inserted. Placement of the inserted gene at a specific locus in the genome is made possible by incorporating it in a vector in which it is flanked by DNA sequences unique to the target site. The artificial genetic material is introduced into an embryo, which then develops into a chimera whose tissues contain both normal cells and cells containing the transgene. Matings among such animals yield some offspring that are homozygous for the transgene. If the inserted gene is a nonfunctional (null) allele, it deletes or "knocks out" the normal, wild allele. Not only is the deleted gene not expressed, but the offspring of matings among homozygous individuals constitute a pure strain, all of whose members lack the gene. Although theoretically any animal could be subjected to the knockout technique, mice have been used almost exclusively. Mice are small and easily maintained and they reproduce rapidly and have a short life span. In addition, mouse and human genomes are strikingly similar, with about 75% correspondence of genes. That knockout mice lacking a wide variety of genes are often phenotypically normal indicates that the mouse genome, like that of human beings, often has sufficient redundancy to compensate for a single missing pair of alleles. Knockout mice lacking the p53 tumor suppressor gene are used in studies of carcinogenesis, and those lacking the gene for the low-density lipoprotein (LDL) receptor constitute an animal model of human familial hypercholesterolemia. Knockout mice have proved valuable in revealing the functions of genes for which mutant strains were not previously available.

A genetically engineered mouse created by gene targeting—targeted gene disruption—in which a specific gene is deleted or inactivated by homologous recombination, to study the effects of its absence. Knockout mouse models of human disease have been created for atherosclerosis, cancer, cystic fibrosis, etc.

knock·out mouse

(nok'owt mows)
A mouse from whose genome a single gene has been artificially deleted.
References in periodicals archive ?
The response to noxious heat stimuli and mechanical stimuli increased obviously in CCI-treated mouse compared with Pirt knockout mouse. Thus, Pirt may modulate neuropathic pain in CCI models by regulation of TRPV1.
In the wriggle mutant mouse, there is a point mutation in the PMCA2 gene resulting in a loss of function phenotype similar to the PMCA2 knockout mouse [13].
Using targeted mutations in transgenic mice, scientists began to develop knockout mouse models of human disease to test the role of specific genes in disease etiology and progression (Eddy 1993; Smithies and Kim 1994).
Lexicon has filed patent applications covering more than 50,000 human gene sequences and more than 35,000 knockout mouse clones and corresponding mouse gene sequences.
This program commenced in 2001 to develop resources in transgenic and knockout mouse models based on single nucleotide polymorphisms (SNPs) in human environmentally responsive genes.
Finally, the 371 patent covers collections of mutated animal cells such as the knockout mouse ES cell clones contained in Lexicon's OmniBank library, as well as Lexicon's gene trapping vectors and related methods.
Lexicon is a leader in defining the functions of genes for drug discovery using large-scale knockout mouse technology.
A companion program, the Comparative Mouse Genomics Centers Consortium, was initiated by the EGP to develop transgenic and knockout mouse models based on human DNA sequence variants in environmentally responsive genes.
At present, Lexicon remains open to negotiation regarding limited sublicenses to companies and institutions that desire to develop in-house knockout mouse research programs.
We have used cDNA microarray analysis of the untreated Cyp1a2(-/-) knockout mouse to search for changes in gene expression that might indicate important intrinsic roles for this enzyme.
One EGP accomplishment of note is the creation of the Comparative Mouse Genome Consortium, which is working to develop transgenic and knockout mouse models based on variants among the environmental response genes.
This initiative is intended to provide the scientific community with new transgenic and knockout mouse models to encourage researchers to study the functional expression of human genetic polymorphisms in the two categories of environmentally responsive genes: DNA repair and cell cycle control.