knock-in


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knock-in

(nok′in″)
An engineered change in a genetic sequence in which an organism's natural gene is replaced by one with a modified nucleic acid sequence that produces a functional protein.
References in periodicals archive ?
With electroporation, the size of the CRISPR gene editing constructs and donor knock-in DNA molecules that are delivered to the target cells are not limited.
Next, using transgenic mice with the G184S Gnai2 allele (knock-ins) which interrupts G-protein [alpha] unit binding to regulators of G-protein signaling (RGS), we found [[alpha].sub.2A]AR antiepileptic effects of EPI were enhanced in hippocampal slices from mutant G[[alpha].sub.0] to mice but not G[[alpha].sub.i2] mice.
In particular, IRES is important for evaluating the success of gene knock-outs or knock-ins in stem cells, which is crucial for the successful creation of transgenic mouse and rat disease models.
"I will be totally fair to them - I won't leave it until the last knock-ins and I'll tell them their futures will be sorted out a lot sooner than that.
Next, using transgenic mice with the G184S Gnai2 allele (knock-ins) which prevents inhibition by interruption of the G-protein alpha unit binding to regulators of G-protein signaling (RGS), we found enhanced [[alpha].sub.2A]AR effects in hippocampal slices from mutant G[[alpha].sub.o] mice but not G[[alpha].sub.i2] mice.
"Chris is progressing nicely and Foley who's just progressing to the last 'knock-ins' of the medial knee ligament," said McCarthy.
"But he's at the last knock-ins of it now - he can run, but it's just kicking a ball.