The red cADDis assay offers an alternative to measurements of cAMP accumulation in cell lysates, because it provides a kinetic measurement
of cAMP and can be targeted to specific populations of cells in mixed cultures.
Microtiter plate reader is a device which allows the endpoint and kinetic measurement
in 96 well microtiter plates according to the actual requirements of the Department of Virology, especially for use in an ELISA method routinely used in the Department of Virology.
We developed a peptide technique involving the use of a triple quadrupole mass spectrometer that allows kinetic measurement
of proteins with relatively low abundance in plasma.
A kinetic measurement
obviates subtraction of background, at the cost of introducing a small positive bias.
Therefore, a combination of diffusion and reaction determines the reaction rate observed for every kinetic measurement
To counter these interferences, analysts have tried many variations of the Jaffe reaction, including modifications of sample preparation, addition of oxidizers to remove interferents, solvent extraction of interferents, pH adjustment of the reaction, continuous-flow dialysis, and kinetic measurement
of the reaction [2-5].
Evaluation of the appropriate kinetic model parameter from kinetic measurement
defines the degree of conversion as a function of time and temperature, [Alpha](T, t).
The instrument is fully automated FelixGX software controls all of the hardware functionality, and provides a full set of data acquisition protocols for spectral and kinetic measurements
The kinetic measurements
were performed spectrophotometrically.
To obtain the kinetic measurements
of overground running the use of a force plate is utilized, where the subject is FORCED to alter gait during their running cycle to allow for readings to be taken (try if for yourself: start running and try to land on a designated fixed point without changing your stride length, cadence or body position) and, as for the treadmill, it's only been very recently that there has been successful development in a generation of treadmills that allows ground reaction force to be measured.
Candidate peptide analytes for kinetic measurements
must contain at least a single leucine residue, have a sequence unique to the target protein in the human plasma proteome, and have properties that are amenable to LC/MS.
They outline the fundamental concepts related to reactor design, such as mixing, unsteady-state operations, multiple steady states, and complex reactions; chemical thermodynamics, with emphasis on the thermodynamics of adsorption and complex reactions; the fundamentals of chemical kinetics, with discussion of microkinetic analysis; heat and mass transfer effects in catalysis, including transport between phases, transfer across interfaces, and the effects of external heat and mass transfer; and tools for making kinetic measurements
and analyzing data.