The red cADDis assay offers an alternative to measurements of cAMP accumulation in cell lysates, because it provides a kinetic measurement
of cAMP and can be targeted to specific populations of cells in mixed cultures.
We developed a peptide technique involving the use of a triple quadrupole mass spectrometer that allows kinetic measurement
of proteins with relatively low abundance in plasma.
The concentration can also be determined by a kinetic measurement
, comparing sample to a calibrator.
Therefore, a combination of diffusion and reaction determines the reaction rate observed for every kinetic measurement
. Diffusion limitations will be most noticeable during the initial part of the reaction and at high conversions.
To counter these interferences, analysts have tried many variations of the Jaffe reaction, including modifications of sample preparation, addition of oxidizers to remove interferents, solvent extraction of interferents, pH adjustment of the reaction, continuous-flow dialysis, and kinetic measurement
of the reaction [2-5].
To gain insight into the photopolymerization process of our dental resin, kinetic measurements
The [H.sub.2][O.sub.2] kinetic measurements
were performed at fixed concentration of Guaiacol (1.33 mM) and [H.sub.2][O.sub.2] concentration varied.
The instrument is fully automated FelixGX software controls all of the hardware functionality, and provides a full set of data acquisition protocols for spectral and kinetic measurements
. HORIBA Scientific www.horiba.com, 732-494-8660
The kinetic measurements
were performed spectrophotometrically.
To obtain the kinetic measurements
of overground running the use of a force plate is utilized, where the subject is FORCED to alter gait during their running cycle to allow for readings to be taken (try if for yourself: start running and try to land on a designated fixed point without changing your stride length, cadence or body position) and, as for the treadmill, it's only been very recently that there has been successful development in a generation of treadmills that allows ground reaction force to be measured.