In the histological analysis of parotid glands, experimental diabetes and/or menopause, acinus epithelial damage, increased lipid droplets, swollen nucleus with karyorrhexis
, eosinophilic stained dense chromatin cells, PMNL cell infiltration and fibrosis in the connective tissue were determined.
These abnormalities included marked binucleation, anisokaryosis, karyolysis, karyorrhexis
Fat necrosis, karyorrhexis
, and admixed histiocytes with vacuolated cytoplasm, containing apoptotic debris and erythrocytes, are commonly seen (Figure 3, A), and this may superficially mimic other reactive conditions, such as lupus.
It includes karyorrhexis
, karyolysis, micronucleus formation, pyknosis, binucleation, broken-egg nucleus, anucleation, etc.
Cytologic atypia included cells that exhibited open chromatin, irregular nuclear contours, prominent nucleoli, or karyorrhexis
. Large aggregates included those greater than 600 gm in greatest dimension.
The only defining morphologic feature seen in patients positive for anti-HLA DSAs was capillaritis, defined as patchy but intense neutrophilic infiltration of pulmonary septae associated with fibrin deposition and neutrophil karyorrhexis
. However, this was only seen in a minority of patients.
From each subject, a minimum of 1000 cells were screened for calculating the frequency of nuclear anomalies which includes multinucleation, binucleation, pyknosis, karyorrhexis
There may be scattered, interposed areas of karyorrhexis
, patchy breakdown, surface repair.
Ishikawa et al (34) based their morphologic assessment of viability on the description in the 2nd edition of Robbins and Cotran Pathologic Basis of Disease, which is similar to the description in the 7th edition: Necrotic (irreversibly injured) cells show increased eosinophilia (acidophilic cytoplasm) and 1 of 3 patterns of nuclear changes--pyknosis, karyorrhexis
, or karyolysis.
Dysplasia Findings Diagnosis 1 RBC: megaloblastoid, 65% RS; t-MDS Meg: small, hypolobated 2 RBC: nuclear irregularity and t-MDS karyorrhexis
, cytoplasmic vacuolization, 8% RS; Myeloid: nuclear atypia, cytoplasmic hypogranularity and vacuolization, pseudo-Pelger-Huet nuclei; Meg: small, hypolobated, clustered 3 RBC: megaloblastoid, nuclear budding, t-AML arising cytoplasmic vacuoles, 52% from t-MDS nucleated RBC precursors Myeloid: blasts with rare granules, no Auer rods Meg: small, hypolobated, clustered Case No.
The resolution is low, and cytologic features such as chromatin texture, presence of pyknosis or karyorrhexis
, nucleolar size, or disorganized cytoskeletal organization cannot be seen.
The cellular variant is defined by the presence of at least one glomerulus with segmental endocapillary proliferation occluding lumina, with or without karyorrhexis
(Figures 4 and 5).