The donor somatic cell karyoplast
is one of the important factors affecting the efficiency of somatic cell animal cloning.
The battle to prevent the transmission of mitochondrial DNA disease: is karyoplast
transfer the answer?
GJ (translation): In our laboratory we used morula blastomeres, because of their differentiating plasticity, as donor karyoplast
57) Researchers then used a karyoplast
fusion technique, using an extract from the Sendai virus, (58) which produced reconstructed oocytes which maintained the spindle-chromosomal complexes transferred from the mature oocyte.
To confirm the enucleation of oocyte, the aspirated karyoplast
in the pipette was visualized under fluorescent light.
Green fluorescent protein (GFP) gene was used as a foreign gene and a non-transfected somatic cell was utilized as a control karyoplast
Our earlier studies using asbestos fiber--treated cytoplasts and fusion with non-treated karyoplasts
provided circumstantial evidence of the role of mitochondria in fiber genotoxicity (Xu et al.
from multinucleated fibroblast cells contain centrosomes and change their morphology to mi totic cells.
DNA hypomethylation of karyoplasts
for bovine nuclear transplantation.
Electrofusion of these oocytes, made of the patient's karyoplasts
and the donor's cytoplasts, resulted in five "reconstructed" zygotes that were transferred into the patient's uterus.
The aspirated karyoplasts
were exposed to ultraviolet-light to confirm the presence of nuclei.